A monovalent form of concanavalin A (m-Con A) has been prepared to determine the importance of valence for human lymphocyte surface binding and subsequent lymphocyte stimulation as measured by blast transformation and cytotoxicity. Concanavalin A (Con A) was fragmented by a proteolytic process and the m-Con A) derivative was isolated by elution with an ascending D-glucose gradient on a Sephadex...

Tumor infiltrating lymphocyte (TIL) is commonly observed in renal cell carcinoma (RCC) tissues. First step of lymphocyte accumulation in cancerous tissues is the lymphocyte migration toward cancer cells. However, no conclusion has been drawn which cytokine is involved in TIL of RCC. The purpose of this study is the identification of a lymphocyte chemotactic factor (chemokine) produced by a newl...

background : now a days researchers have shown grean interest in electromagnetic waves which spread from devices such az microwaves,satellites,tv,cell phones etc,due to various reports of their devasting effects on animal growth and health.in this study the effects of these radiations on blood cells is investigated . methods : in the present study the effect of radiation on blood parameters in ...

background coronary heart disease (chd) is the leading cause of death worldwide. research indicates that coronary atherosclerosis is the most frequent cause of chd. evidence is scarce concerning the clinical efficacy of fibrinogen or neutrophil-lymphocyte ratio (nlr) measurement in risk-stratifying patients with chronic stable angina. conclusions nlr and fibrinogen predicted cad, independent of...

Perfluorinated compounds (PFCs) such as perfluorooctanesulfonate (PFOS) are stable chemicals that accumulate in biological matrix. Toxicity of these compounds including immunotoxicity has been demonstrated in experimental models and wildlife. Although limited number of studies examined the effects of PFOS on human lymphocytes but so far no research has investigated the complete mechanisms of PF...

In vitro studies were performed to determine the proliferative responsiveness of human peripheral blood thymus-dependent (T) and thymus-independent (B) lymphocytes to phytomitogens and allogeneic lymphocytes. Recombination of T and B cells, with selective inhibition of proliferation of one of the two populations, was used to identify cellular interactions which may contribute to cell proliferat...

Interferon-alpha inducers were previously shown to cause human lymphocyte production of a corticotropin (ACTH)-like peptide. Thyrotropin (TSH) was not produced under these conditions. In contrast, this report shows that a T-cell mitogen (staphylococcal enterotoxin A), which does not induce the ACTH-like peptide, caused human lymphocyte production of an immunoreactive (ir) TSH. Lymphocyte synthe...

This study evaluated direct and metabolic genotoxic effects caused by Lannate-90®, a methomyl-based formulation (90 % active ingredient), in human lymphocyte cultures using sister chromatid exchange assay (SCE). Two processes were used for the plant promutagens evaluation: in vivo activation, applying the insecticide systemically in plants for 4 h and subsequently adding plant metabolites conta...

background: similar to inflammatory cells, peripheral blood mononuclear cells (pbmcs) can also infiltrate in to kidney and urinary tracts and subsequently excreted by urine. in this study we determined the viability rate and response to phytohemagglutinin-a (pha) of human pbmcs in normal urine. methods: a number of 1×106 ficoll-hypaque isolated pbmcs were dispensed in 1 ml normal urine and 6 mo...

INTRODUCTION Gene therapy could prevent bleeding in hemophilia. However, antibodies could inhibit coagulation, while cytotoxic T lymphocytes could destroy modified cells. The immaturity of the newborn immune system might prevent these immune responses from occurring after neonatal gene therapy. MATERIALS AND METHODS Newborn dogs, cats, or mice were injected intravenously with a retroviral vec...