Enveloped viruses employ diverse and complex strategies for wrapping at cellular membranes, many of which are poorly understood. Here, an ultrastructural study of herpes simplex virus 1 (HSV1)-infected cells revealed envelopment in tubular membranes. These tubules were labelled by the fluid phase marker horseradish peroxidase (HRP), and were observed to wrap capsids as early as 2 min after HRP ...

background: herpes simplex virus type 1 is one of the most common viruses among human population. studies demonstrate the essential role of cell mediated immunity, especially cd8+ t cells, in prevention and clearance of hsv1. objective: it is of great importance to improve our knowledge about the kinetics of ctl responses to primary and secondary hsv-1 infection. methods: using a sensitive tech...

To analyze the subcellular trafficking of herpesvirus capsids, the small capsid protein has been labeled with different fluorescent proteins. Here, we analyzed the infectivity of several HSV1(17(+)) strains in which the N-terminal region of the non-essential small capsid protein VP26 had been tagged at different positions. While some variants replicated with similar kinetics as their parental w...

695 There are now 9 human herpesviruses (HHVs); the increase from 8 to 9 was caused by a recent decision to split HHV strains 6A and 6B into distinct species. The 9 species are subdivided into 3 subfamilies: alpha (herpes simplex virus [HSV] types 1 and 2 and varicella-zoster virus [VZV]), beta (cytomegalovirus, HHV6A, HHV6B, and HHV7), and gamma (Epstein-Barr virus and Kaposi sarcoma–associate...

Clinical Validation of the Lyra Direct HSV1+2/VZV Assay for Simultaneous Detection and 1 Differentiation of Three Herpesviruses in Cutaneous and Mucosal Lesions 2 3 4 Feinan Fan , Jeffrey Stiles, Albina Mikhlina, Xuedong Lu, N. Esther Babady, and Yi-Wei 5 Tang 6 7 8 1 Department of Laboratory Medicine, Futian Hospital, Guangdong Medical College, Shenzhen, 9 China; 2 Department of Laboratory Med...

Rubber surgical gloves worn as a barrier to prevent contamination from body fluids offer relative protection against contamination through direct percutaneous injuries involving needles, scalpel blades or bone fragments. To determine the main experimental parameters influencing the volume of blood transmitted by a hollow-bore needle (worst case scenario) during an accidental puncture, we design...

The ability to monitor the responses of and inhibit the growth of brain tumors during gene therapy has been severely limited due to the blood-brain barrier (BBB). A previous study has demonstrated the feasibility of noninvasive in vivo imaging with 123I-2'-fluoro-2'-deoxy-5-iodo-1-β-D-arabinofuranosyluracil (123I-FIAU) for monitoring herpes simplex virus type 1 thymidine kinase (HSV1-tk) cancer...