نتایج جستجو برای: hrp immunoassay

تعداد نتایج: 20751  

Journal: :زیست شناسی جانوری تجربی 0

abstract peroxidases are a group of oxidoreductases that are produced by a number of microorganisms and plants, and catalyse the reduction of peroxides. peroxidases are widely used in clinical biochemistry and enzyme immunoassay. horseradish peroxidase isoenzyme c (hrp) is one of the characterized peroxidases. the structure of the enzyme is largely alpha helical. kinetics studies of peroxidase ...

2013
Sadhanandam Preethi Ayyappan Anumary Meiyazhagan Ashokkumar Palanisamy Thanikaivelan

Biocatalysis based effluent treatment has outclassed the presently favored physico-chemical treatments due to nil sludge production and monetary savings. Azo dyes are commonly employed in the leather industry and pose a great threat to the environment. Here, we show the degradation of C. I. Acid blue 113 using horseradish peroxidase (HRP) assisted with H2O2 as a co-substrate. It was observed th...

1997
Seema Jaggi

This thesis describes a new adaptive approximation technique called high resolution pursuit (HRP), and demonstrates how HRP can be used to extract features that are suitable for object recognition. Recently, adaptive approximation techniques have become popular for obtaining representations of large classes of signals. These techniques include method of frames, matching pursuit, and basis pursu...

Journal: :Biotechnology and bioengineering 2001
B Morawski S Quan F H Arnold

Biotechnology applications of horseradish peroxidase (HRP) would benefit from access to tailor-made variants with greater specific activity, lower K(m) for peroxide, and higher thermostability. Starting with a mutant that is functionally expressed in Saccharomyces cerevisiae, we used random mutagenesis, recombination, and screening to identify HRP-C mutants that are more active and stable to in...

2018
Suzanne M Huddy Inga I Hitzeroth Ann E Meyers Brandon Weber Edward P Rybicki

Horseradish peroxidase (HRP) is a commercially important reagent enzyme used in molecular biology and in the diagnostic product industry. It is typically purified from the roots of the horseradish (Armoracia rusticana); however, this crop is only available seasonally, yields are variable and often low, and the product is a mixture of isoenzymes. Engineering high-level expression in transiently ...

2016
Lijun Yan Xiaofeng Wang Qiutong Li Wencheng Wang Shixing Gong Zheng You Peng Yi Wei Sun

In this paper nickel oxide (NiO) nanoflower was synthesized and used for the realization of direct electrochemistry of horseradish peroxidase (HRP). By using carbon ionic liquid electrode (CILE) as the substrate electrode, NiO-HRP composite was casted on the surface of CILE with chitosan (CTS) as the film forming material and the modified electrode was denoted as CTS/NiO-HRP/CILE. UV-Vis absorp...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تربیت مدرس - دانشکده علوم پزشکی 1389

هدف: آفلاتوکسین ها و بخصوص آفلاتوکسین b1، اثرات مرگ بار و جبران ناپذیری روی سلامت انسان دارند. هدف از انجام این تحقیق، توسعه یک روش سریع، ساده، حساس و اختصاصی برای شناسایی و اندازه گیری آفلاتوکسین متصل به آلبومین در سرم خون بعنوان شاخص مهم وضعیت در معرض بودن افراد به این سم می باشد. مواد و روش ها: در این تحقیق سه گروه رات انتخاب و به عنوان گروه تست (تیمار شده با آفلاتوکسین (b1، گروه کنترل من...

Journal: :The Journal of Cell Biology 1984
P J Courtoy J Quintart P Baudhuin

Galactosylated BSA (galBSA) and its conjugate to horseradish peroxidase (galBSA-HRP) enter the galactose-specific pathway of hepatocytes. 10 min after intravenous injection, structures containing either ligand sediment mostly between 33,000 and 3 X 10(6) g X min (LP fraction) and have an equilibrium density of 1.11-1.13 g/ml in sucrose gradients (Quintart, J., P. J. Courtoy, J. N. Limet, and P....

Journal: :The Biochemical journal 2002
Frank Dietz Sebastian Franken Kenya Yoshida Hideji Nakamura Joachim Kappler Volkmar Gieselmann

Hepatoma-derived growth factor (HDGF)-related proteins (HRPs) comprise a family of polypeptides named after HDGF, which was identified by its mitogenic activity towards fibroblasts. In the present study, we describe a hitherto unknown HRP, termed HRP-4. The cDNA of bovine HRP-4 (bHRP-4) predicts a polypeptide of 235 amino acids. Northern- and Western-blot analyses of various bovine tissues demo...

Journal: :Journal of Science and Medicine in Sport 2021

Background: Hip-related pain (HRP) is a movement-related condition; however, previous biomechanics research into HRP have concentrated on male-dominated cohorts and focused people undergoing surgical intervention. As result, little known about the biomechanical impairments of women with those not seeking surgery. Therefore, aims this study were threefold: (i) to evaluate differences in lower-li...

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