In this study, we used a recently developed nuclear magnetic resonance (NMR) technique to measure ionized calcium in sickle erythrocytes. The NMR technique, which involves 19F NMR studies of a fluorinated calcium chelator quinMF, [2-(2-amino-4-methyl-5-fluorophenoxy)methyl-8-aminoquinoline-N,N,N',N'- tetraacetic acid] provides a novel approach to the study of ionized calcium in erythrocytes sin...

INTRODUCTION The medical management of secondary hyperparathyroidism in patients with end-stage renal disease involves a combination of dietary restrictions, phosphate binders, active vitamin D analogs, and calcimimetics. CASE PRESENTATION We report the case of a 36-year-old Hispanic dialysis patient, originally from Cuba and now residing in the USA, who developed severe bone pain and muscle ...

A small quantity of extracellular calcium is required for the stimulation of lymphocytes by mitogens such as plant lectins. Lectin binding to the lymphocyte surface and early postbinding events that eventually lead to DNA synthesis are calcium dependent. Mitogenic lectins such as PHA and Con-A rapidly increase the size of an exchangeable pool of cell calcium and cause a smaller rise in intracel...

OBJECTIVE To determine if the intraoperative rapid parathyroid hormone (PTH) assay can be used to accurately predict postoperative calcium levels following total or completion thyroidectomy. DESIGN A prospective study. SETTING Tertiary care referral center. PATIENTS One hundred four patients following a total or completion thyroidectomy.Intervention Intraoperative rapid plasma PTH levels ...

The primary role of vitamin D is regulation of calcium, phosphorus and bone metabolism. Vitamin D status assessment is based on measuring of 25 (OH) D concentrations, and disorders of vitamin D status may be manifested as a vitamin D insufficiency, vitamin D deficiency and vitamin D hypervitaminosis. It is generally accepted that values above 75 nmol / L will suffice to prevent the occurrence o...

The long-term clinical effects of the use of a low calcium concentration in the dialysate are largely unknown. For this reason, the influence of low-calcium dialysate on parathyroid hormone (PTH) secretion in hemodialysis patients and its long-term effect on the severity of secondary hyperparathyroidism were studied. In 35 hemodialysis patients, the dialysate calcium concentration was lowered f...

We measured the activity of Ca2+, Na+, K+, and Cl- with ion-selective electrodes after equilibrium dialysis of solutions with different albumin concentrations. The calculated Donnan ratio was the same for all ions in the same solution and increased with the albumin concentration, as predicted by the Donnan theory. The Donnan distribution ratio for Ca2+ was similar, as determined with instrument...

Although the toxic effects of citrate including hemodynamic and cardiovascular changes result from a decrease in ionized calcium levels in serum due to chelating action, these effects of citrate on blood coagulation have not yet been fully clarified. The present study examines whether serum citrate and ionized calcium levels affect whole blood clotting time in rats using the test tube method in...

Hypercalcaemia is often thought to provide important clues to the presence of unsuspected illness. A presumptive diagnosis of hypercalcaemia may often be made on an elevated total serum calcium concentration although it should, strictly speaking, refer to an elevation in the physiologically important ionized (free) calcium concentration. Pseudohypercalcaemia is rare, but should be excluded befo...