Genetic disorders of homocysteine (Hcy) or folate metabolism or high-methionine diets elevate plasma Hcy and its atherogenic metabolite Hcy-thiolactone. In humans, severe hyperhomocysteinemia due to genetic alterations in cystathionine beta-synthase (Cbs) or methylenetetrahydrofolate reductase (Mthfr) results in neurological abnormalities and premature death from vascular complications. In mous...

A BrPAPS based Cu 2+ complex has been developed as a colorimetric probe for the selective recognition of homocysteine (Hcy) over cysteine (Cys) and glutathione (GSH) in an aqueous solution via indicator displacement assay. formed with 1:1 ratio (BrPAPS-Cu ) accompanied by color change from yellow to red. Detecting Hcy is on high affinity . The addition BrPAPS-Cu caused formation 2:1 stoichiomet...

Elevated plasma levels of homocysteine (Hcy), known as hyperhomocysteinemia (HHcy), are associated with osteoporosis. A decrease in bone blood flow is a potential cause of compromised bone mechanical properties. Therefore, we hypothesized that HHcy decreases bone blood flow and biomechanical properties. To test this hypothesis, male Sprague-Dawley rats were treated with Hcy (0.67 g/L) in drinki...

Increased oxidative stress, alterations of lipid metabolism and induction of thrombosis have been suggested to be pathogenic links which are present between hyperhomocysteinaemia and atherosclerosis. However, the mechanism by which homocysteine (Hcy) can promote atherogenesis is far from clear and it has been debated. In the presence of cardiovascular risk factors, endothelial dysfunction is th...

Elevated levels of homocysteine (hcy) form a vascular risk factor. It has been shown that hcy induces cell death in vascular smooth muscle cells (smcs). Recent studies have shown that nadph oxidase (nox)-mediated reactive oxygen species (ros) play a role in hcy-induced apoptosis in cardiomyocytes and endothelial cells. In this study we have analyzed the role of hcy on the different nox isoforms...

OBJECTIVE We previously reported that homocysteine (Hcy) inhibits endothelial cell (EC) growth and promotes vascular smooth muscle cell (VSMC) proliferation. This study characterized and directly compared Hcy transport in cultured human aortic ECs (HAECs) and smooth muscle cells (HASMCs). METHODS AND RESULTS Hcy (10 micromol/L) was transported into both cell types in a time-dependent fashion ...

High homocysteine (HCy) levels are associated with lymphocyte-mediated inflammatory responses that are sometimes in turn related to hypoxia. Because adenosine is a potent lymphocyte suppressor produced in hypoxic conditions and shares metabolic pathways with HCy, we addressed the influence of high HCy levels on the hypoxia-induced, adenosine-mediated, alteration of lymphocyte viability. We trea...

BACKGROUND Hyperhomocysteinemia and the metabolic syndrome are established cardiovascular risk factors and are frequently associated with hypertension. The relationship of plasma homocysteine (Hcy) with the metabolic syndrome and insulin resistance, however, is debated and studies in hypertensive patients are limited. In this study, we have investigated the association of Hcy with the metabolic...

Background: Hyperhomocysteinemia is a recognized risk factor for atherosclerosis. Elevated homocysteine (Hcy) results in severe oxidative stress, platelets hyper activity, and impairs arterial circulation and smooth muscle proliferation in vascular wall. This study was carried out to evaluate follicular fluid (FF) Hcy, glucose and estradiol levels in IVF candidate PCOS women. Materials and Meth...