Masses of homogeneous electron-dense material accumulate in the cytoplasmic inclusions of cultured fibroblasts which have been infected with "wild" and "adapted" strains of human cytomegalovirus. The substance appears to be produced by microtubular membranes and the Golgi apparatus; ultrastructural histochemistry suggests that it is not lysosomal in nature nor is it comprised of lipids or polys...

Problems of lignin formation have long occupied the attention of investigators, however, neither the physiological nor chemical mechanisms involved in lignification is known in detail. Nevertheless, a considerable body of knowledge has accumulated through the investigations of Freudenberg, Nord, Brown, Wardrop, and others (4, 5, 6, 10, 13, 15, 23), especially on the biochemistry, histochemistry...

Abnormal fucosylation of cystic fibrosis mucin was previously shown using peroxidase conjugated lectins on ileal tissue sections. These abnormally fucosylated glycoproteins were investigated further using monoclonal antibodies to fucosyl oligosaccharides based on type 1 and type 2 blood group precursor chains. The results of this study, using monoclonal antibodies to blood group glycoproteins i...

Two cases suffering from the syndrome of `continuous muscle-fibre activity' have been followed-up for 14 years. These patients have gradually gone into remission and no longer require therapy. The results of recent histology, histochemistry, and electronmicroscopy, as well as sural nerve biopsy studies, are presented. The sarcoplasmic reticulum calcium binding activity and ATPase activity are n...

Primates are frequently used today in dental research and in South Africa the primate most commonly used is the vervet monkey (Cercopithecus pygerythus). This paper describes the normal histology of the soft palate in this animal based on a preliminary study towards the possible use of the vervet monkey in cleft palate research. Materials and methods Twenty-five soft palates from adult vervet m...

Found at all depths and latitudes, sponges are among the most ubiquitous of marine organisms. With the advent of modern laboratory and field techniques (e.g., electron microscopy, histochemistry, the development of molecular biology and biochemistry, and the use of SCUBA for collection purposes) our knowledge of sponge biology has mushroomed (see reviews by Fell, 1974; Bergquist et al., 1979). ...

Histochemical studies performed on livers of the bluegill, Lepomis macrochirus, containing the larval cestode, Proteocephalus sp., indicated that the cellular capsule surrounding the parasite was composed of glycogen, basic protein with large concentrations of protein-bound amino groups, and tyrosine. An amorphous layer between the capsule and parasite gave positive reactions for mucoproteins, ...

Barium granuloma of the colon and rectum is a rare complication of X-ray examination of the digestive tract using barium. The authors report 5 new cases occurring in the last 3 years. Histological examination revealed a granulomatous reaction with greyish finely granular refractile PAS-negative material located in the cytoplasm of histiocytes and in the interstitial space. The radiographic stud...

The histochemical and ultrastructural analysis of the nuclear components involved in RNA transcription and splicing can reveal the occurrence of cellular dysfunctions eventually related to the onset of a pathological phenotype. In recent years, nuclear histochemistry at light and electron microscopy has increasingly been used to investigate the basic mechanisms of skeletal muscle diseases; the ...

Our previous study reported the rich existence of multinucleated giant cells in an autoimmune myocarditis experimentally induced in rats. The present study investigated the histochemical and ultrastructural characteristics of these giant cells. Histochemistry for an acid phosphatase clearly demonstrated multinucleated giant cells dispersed at the inflammatory foci. Ultrastructurally, the giant ...