this article is to analyze the tendency of mirza fath’ali akhoondzadeh to freemasonic circle, his relations to the members of mirza malkam khan’s freemasonry center and his playwriting on the basis of such relations. initially, his tendency to freemasonic cycles in tbilisi and his relations to malkam khan’s freemasonry center have been proved and   his common intellectual elements with malkom k...

The research aimed to determine the sorts and most prevalent illocutionary acts used by characters in Brave animated movie. act was defined as purpose for which speaker utters something. focused on main character entire conversation between each applied a qualitative method where data were taken from script uttered all of movie who acts. collection technique done five steps; made analyzing form...

We begin by thanking our discussants, Jiwoong Shin and K. Sudhir (2009) and Luís Cabral (2009), for their thoughtful comments on our research (Dubé, Hitsch, and Rossi 2009). Both commentaries focus on the stylized (“simple”) model in our article, which we used to motivate our more general empirical model. Shin and Sudhir solve a two-period Hotelling model. They find a U-shaped relationship betw...

This study establishes a theoretical framework and provides empirical evidence related to the motivations and benefits sought by store-loyal customers. From a theoretical perspective, the proposed framework distinguishes utilitarian benefits, such as monetary and time savings, from hedonic benefits, such as shopping enjoyment, inno-vativeness and impulsiveness. From a methodological perspective...

BACKGROUND A versatile strategy for protein-surface coupling in biochips exploits the affinity for polyhistidine of the nitrilotriacetic acid (NTA) group loaded with Ni(II). Methods based on optical reflectivity measurements such as spectroscopic ellipsometry (SE) allow for label-free, non-invasive monitoring of molecule adsorption/desorption at surfaces. RESULTS This paper describes a SE stu...

Here we describe a generic procedure for the expression and purification of milligram quantities of functional recombinant eukaryotic integral membrane proteins, exemplified by hexahistidine-tagged bovine rhodopsin. These quantities were obtained with the recombinant baculovirus/Sf9 insect cell-based expression system in large-scale bioreactor cultures with the use of a serum-free and protein-f...

The crystal structure of a mutant form of the single-chain fragment (scFv), derived from the monoclonal anti-His tag antibody 3D5, in complex with a hexahistidine peptide has been determined at 2.7 A resolution. The peptide binds to a deep pocket formed at the interface of the variable domains of the light and the heavy chain, mainly through hydrophobic interaction to aromatic residues and hydr...

The cDNA of the human GM2-activator protein was cloned into the expression vector pHX17. The plasmid encodes a fusion protein with a hexahistidine tail and a Factor Xa cleavage site at its N-terminus. The recombinant protein was purified from cell homogenates under denaturing conditions by metal-ion affinity chromatography in a single step and then was refolded. The hexahistidine tail could be ...

We report oriented immobilization of proteins using the standard hexahistidine (His6)-Ni2+:NTA (nitrilotriacetic acid) methodology, which we systematically tuned to give control of surface coverage. Fluorescence microscopy and surface plasmon resonance measurements of self-assembled monolayers (SAMs) of red fluorescent proteins (TagRFP) showed that binding strength increased by 1 order of magni...

The fungal pathogen Colletotrichum lindemuthianum secretes an endo-chitin de-N-acetylase (ClCDA) to modify exposed hyphal chitin during penetration and infection of plants. Although a significant amount of biochemical data is available on fungal chitin de-N-acetylases, no structural data exist. Here we describe the 1.8 A crystal structure of a ClCDA product complex and the analysis of the react...