Elaboration of different toxins by enterotoxigenic E. coli has been considered as one of the main virulence factors contributing to the manifestation of disease caused by these microorganisms. Various strategies have been employed to raise antibodies against these toxins as a line of defense. In this study, the 3’ terminus of the gene that codes for the binding subunit of the heat-labile entero...

The insertion of multiple copies of DNA elements into plasmid vectors is frequently used to amplify the functions of the element. For example, tandem repeat elements can generate a hyperactive bait in yeast one-hybrid systems (3), and multiple copies of enhancer elements can greatly increase promoter strength (8). The latter effect may be important in vector development for transgenic animals a...

infectious bronchitis (ib) disease is one of the important respiratory diseases of poultry that causes annually large economic losses in poultry industry of iran. the aim of this study is molecular characterization of s1 gene of iranian infectious bronchitis viruse (ibv) belonged to 793/b serotype. the whole s1 gene of three local ibv strains in rt-pcr, showed a band above 1600 base pair (bp) i...

a total of 105 isolates of erysiphe betae from different regions of iran were investigated. for probable polymorphism of its-5.8s regions using pcr-based rflp, the mentioned fragments were amplified via pcr by its1 and its4 primers and subjected to 1.5% agarose gel electrophoresis. pcr products were digested by haeiii, alui, taqi, hindiii, cfoi, ecori, mspi, saci and rsai res and the digestion ...

A lambda gt11 expression library of Tn5-tagged invasion plasmid pWR110 (from Shigella flexneri serotype 5, strain M90T-W) contained a set of recombinants encoding a 60-kilodalton protein (designated IpaH) recognized by rabbit antisera raised against S. flexneri invasion plasmid antigens (J. M. Buysse, C. K. Stover, E. V. Oaks, M. M. Venkatesan, and D. J. Kopecko, J. Bacteriol. 169:2561-2569, 19...

Serotyping of clinical isolates is a widely used technique for epidemiologic study of group B streptococcal infections. However, serotyping cannot definitively determine epidemiologically related or unrelated isolates. We investigated the use of restriction endonuclease analysis (REA) with both conventional agarose gel electrophoresis (AGE) and pulsed-field gel electrophoresis (PFGE) in 50 isol...

Virulent Rhodococcus equi, which is a well-known cause of pyogranulomatous pneumonia in foals, possesses a large plasmid encoding virulence-associated 15- to 17-kDa antigens. Foal and soil isolates from five countries-Argentina, Australia, Canada, France, and Japan-were investigated for the presence of 15- to 17-kDa antigens by colony blotting, using the monoclonal antibody 10G5, and the gene c...