Miconazole, a broad-spectrum antimycotic agent with some antibacterial activity, has recently become available for experimental parenteral use in the United States. Its efficacy as an anticandidal drug was tested in adult Wistar rats. A previously established infectious dose of 5 x 10(6)Candida albicans was intravenously injected into 250- to 300-g animals. This dose was fatal to 95% (20/21) of...

BACKGROUND AND OBJECTIVES Pneumocystis jirovecii pneumonia (PJP) is a chronic fungal infection that caused by P. jirovecii. Disease is more prevalent among the HIV-infected patients. The colonization of pneumocystis in human respiratory system is associated with the airway inflammation and obstruction. The current study was conducted to identify the prevalence of P. jirovecii among the patients...

BACKGROUND Histoplasmosis (HP) is diagnosed by visualizing intracellular microorganisms in biopsy and/or culture. Periodic-acid Schiff (PAS) and Gomori methenamine silver (GMS) staining methods are routinely used for identification. The acid-fast property of Histoplasma was identified decades ago, but acid-fast staining has not been practiced in current surgical pathology. Awareness of the acid...

Age related cytochemical changes of thymic endocrine cells were studied in 78 day old chicks at five day interval to age of day 60 employing a panel of cytochemical stains. Methenamine silver revealed cell morphology including cell processes distinctly while diamine silver revealed a stronger argentaffinity in these cells. The cells had greater affinity for diamine silver compared to methenamin...

AIMS To compare the efficacy of histochemical and immunohistochemical methods in detecting forms of Sporothrix schenckii in tissue. METHODS Thirty five cutaneous biopsy specimens from 27 patients with sporotrichosis were stained by histochemical haematoxylin and eosin, periodic acid Schiff, and Gomori's methenamine silver methods and an immunohistochemical (avidin-biotin complex immunoperoxid...

The distribution of lactoferrin and glycoprotein in human bronchial glands has been studied by electron microscopy using an immunoperoxidase method to stain the former and a periodic acid-chromic acid-silver methenamine sequence for the latter, each applied to ultrathin Epon sections. The distribution of lactoferrin corresponds to that of lysozyme. Lactoferrin and lysozyme are both confined to ...

We are reporting a rare case of primary mucocutaneous histoplasmosis. A 47-year-old male with a part-time job of rearing pigeons presented with a non-healing oral ulcer with no associated systemic symptoms. Differential diagnosis of oral ulcerative lichen planus, squamous cell carcinoma and mucocutaneous leishmaniasis was considered. Histopathology showed multiple organisms with a peripheral ha...

The enterochromaffin cells of the guinea pig duodenum and 5-hydroxytryptamine incorporated into gelatin models gave, after formalin fixation, nearly identical reactions with methenamine silver, ferric-ferricyanide, and diazotized o-aminoazotoluene. Following formaldehyde fixation the indole reaction with p-dimethylaminobenzaldehyde was negative in tissue enterochromaffin cells; similarly the in...

In 1965, it was observed that patients with glomerulonephritis with persistently low serum concentrations of C3 evolved to have similar glomerular morphologic characteristics, particularly recognizable in biopsy specimens stained by the Jones methenamine silver technique [1]. Subsequent observations have indicated that although long-standing hypocomplementemia is commonly observed and is unique...

1 Mones JM, Saldana MJ, Oldham SA. Diagnosis of Pnewnocysti.s carinii pneumonia. Roentgenographic-pathologic correlates based on fiberoptic bronchoscopy specimens for patients with the acquired immunodeficiency syndrome. Chest 1986; 89:522-26 2 Hartman B, Koss M, Hui A, Baumann W, Athos L, Boylen T Pneumocystis carinii pneumonia in the acquired immunodeficiency syndrome (AIDS). Diagnosis with b...