Groups of rats bearing Morris minimal deviation hepatoma 7288CTC were fed a fat-free diet supplemented with either 0.5% safflower oil (diet A), 15% safflower oil or free acids (diets Band C), or 15% safflower oil or free safflower fatty acids (diet D) for 4 weeks. A group of normal rats was also fed diet D. Triglycerides, cholesteryl esters, phosphatidylcholines, and phosphatidylethanolamines i...

The pathway of fat oxidation in two experimental hepatomas was studied in order to demonstrate that a specific deficit in the energy metabolism of a tumor might contribute to the cachexia of the host. Forty-eight male Buffalo rats were divided into four groups of 12 each. One group was implanted s.c. with Morris hepatoma 7777 and one group was implanted with Morris hepatoma 7800, whereas the ot...

A panel of six murine monoclonal antibodies against hepatocellular carcinoma-associated antigens, reactive with PLC/PRF/5 human hepatoma cells, was conjugated to Adriamycin (doxorubicin) via a dextran bridge. This library of antibodies includes three monoclonal antibodies against hepatitis B virus surface antigen, one anti-alpha-fetoprotein, and two other IgG2a antibodies against PLC/PRF/5 hepa...

The degradation rates of N-acetylneuraminic acid (NANA) were investigated in plasma membranes and in the total protein of rat liver and Morris hepatoma 7777. The plasma membranes of Morris hepatoma 7777 could not be isolated by known methods useful for isolation of rat liver plasma membranes. Therefore, a modified method was developed. The specific activity of 5'-nucleotidase as a plasma membra...

The major cell type supporting hepatitis C virus (HCV) infection is the hepatocyte; however, most reports studying viral entry and replication utilize transformed hepatoma cell lines. We demonstrate that HCV pseudoparticles (HCVpp) infect primary hepatocytes with comparable rates to hepatoma cells, demonstrating the limited variability in donor hepatocytes to support HCV receptor-glycoprotein-d...

In an effort to decrease the toxicity of camptothecin (CPT) and improve selectivity for hepatoma and colon cancer cells, bile acid groups were introduced into the CPT 20 or 10 positions, resulting in the preparation of sixteen novel CPT-bile acid analogues. The compounds in which a bile acid group was introduced at the 20-hydroxyl group of CPT showed better cytotoxic selectivity for human hepat...

HYBRIDS FROM A CROSS OF RAT HEPATOMA CELLS WITH DIPLOID EPITHELIAL CELLS FROM RAT LIVER HAVE BEEN STUDIED WITH RESPECT TO KARYOTYPE AND EXPRESSION OF TWO FUNCTIONS LIMITED TO THE HEPATOMA PARENT: high level of the enzyme tyrosine aminotransferase (EC 2.6.1.5; L-tyrosine:2-oxoglutarate aminotransferase) and its inducibility with steroid hormones. The hybrids that contain the complete chromosomal...

The present study investigated the effect of dietary soy protein isolate (SPI) on tumor necrosis factor-alpha (TNF) productivity in peritoneal macrophages from nephritic and hepatoma-bearing rats. Dietary SPI significantly inhibited the elevated production of TNF by lipopolysaccharide-stimulated macrophages in nephritic and hepatoma-bearing rats compared with dietary casein, while it exerted no...

The distribution of sucrose and inulin has been compared with that of radio-iodihated serum albumin in slices of normal rat liver and of hepatomas produced with dimethylaminoazobenzene. I t was found that the radio-iodinated serum albumin and inulin spaces are both larger in hepatomas than in normal liver, whereas the sucrose space is larger in liver than in hepatoma. The change in volume of li...

Chromatin from two Syrian hamster tissues: the Kirkman-Robbins hepatoma and the liver, has been separated into soluble (S) and insoluble (P) fractions. Both fractions contain the complete set of five main histones but differ in respect of HI subfractions. The hepatoma chromatin is known to contain an unusual HI subfraction, HI slow [12, 13], probably identical with a similar subfration present ...