background diabetes mellitus as a chronic metabolic disease occurs in patients with partial or complete deficiency of insulin secretion or disorder in action of insulin on tissue. the disease is known to provide conditions for overgrowth of candida species. candida spp. cause candidiasis by many virulence factors such as esterase, hemolysin and phospholipase. objectives this study aimed to comp...

staphylococci isolated from 98 mastitic cow’s milk samples were analyzed for the production of alpha,beta and delta-hemolysin on sheep, bovine and horse red blood cells and in vitro susceptibility to several antimicrobial agents. out of a total of 18 isolated strains, 100% demonstrated hemolytic activity either alone or in combined forms. among 8 isolates of s. aureus 3 isolates produced α/β an...

The iron uptake systems of Plesiomonas shigelloides strains were determined. Siderophore production was not detected by chemical or biological assays, and the strains tested were unable to use enterobactin, aerobactin, or vibriobactin for growth in low-iron media. Both hemin and hemoglobin supported full growth of the bacteria in media lacking other iron sources, but neither transferrin nor lac...

Brachyspira hyodysenteriae is the primary cause of swine dysentery, which is responsible for major economic losses to the pig industry worldwide. The hemolytic activity of 10 B. hyodysenteriae strains isolated from stools of pigs with mild to mucohemorrhagic diarrhea was compared and seven hemolysis associated genes were sequenced. Hemolysis induced by these strains varied from strong to near a...

By CARL F. HINz, JR. T HE HEMOLYSIN of the Donath-Landsteiner reaction, responsible for [)arOxysmal cold hemoglobinuria, has not been characterized physicochemically. Other erythrocyte agglutinins and hemolysins have been identified in both the 75 and 195 fractions of y-globulin,1 and certain other of the cold antibodies have been identified with the 195 globulins.2 Further, the Donath-Landstei...

Heat-stable hemolysin produced by the cellophane-agar plate method consisted of two major acidic glycolipids. One was composed of 2 mol of rhamnose and 2 mol of beta-hydroxydecanoic acid, and the other was composed of 1 mol of rhamnose and 2 mol of beta-hydroxydecanoic acid. The hemolysis-producing moiety of the hemolysin was shown to be the dimer of beta-hydroxydecanoic acid contained in the g...

A multiplex PCR was developed for detection of hemolysin-producing Vibrio anguillarum using primers targeting five hemolysin genes (vah1, vah2, vah3, vah4 and vah5). This method was successful in amplifying reactions containing as little as 100 fg of genomic template DNA. The direct detection of V. anguillarum in clinical specimens by this multiplex PCR was also successful in reactions containi...

possibly the formation of methemoglobin, had taken place in the blood cells. This raises the question whether the effect observed is due to hemolysin production by S. bovis or whether the blood cells are perhaps altered by CO2 in such a way as to render them susceptible to some other chemical reaction of the bacteria. For this reason the authors have refrained from ascribing this effect to a he...