A new latex agglutination test for rubella virus was used to test sera with a hemagglutination inhibition titer of -8 (97 specimens) or >256 (158 specimens). Maximum latex agglutination test sensitivity was achieved when low-titer sera were tested undiluted and high-titer sera were diluted (1:10). A modification of the protocol of the manufacturer resulted in a latex agglutination-hemagglutinat...

Treatment of sheep erythrocytes with suitable concentrations of tannic acid render them capable of adsorbing certain protein molecules from solution in saline. Red cells which have adsorbed proteins in this way are agglutinated after washing by the homologous antiprotein sera, even by high dilutions. Through hemagglutination sera can be titrated for antibodies against antigens adsorbed on the c...

A hemagglutination reaction has been described between sheep erythrocytes treated with a component of a polysaccharide fraction of mammalian tubercle bacilli and the sera of experimental animals or of tuberculous patients. Evidence has been presented for the specificity of this reaction. A modification of the test, involving an inhibition reaction, has been developed for the detection and quant...

The Present study demonstrates the diagnostic characteristics of Pedilanthus tithymoloides, agglutinin (PTA), lectin based hemagglutination method for screening of thyroid disorders as compared with RIA as gold standard. The result estimates 90% sensitivity, 95.55% specificity and 92.63% observed agreement with RIA test in thyroid disorders. The result of hyperthyroidism, hypothyroidism and cre...

Antibody production after injection of Mycobacterium tuberculosis was studied by use of the single cell plaque assay technique. Maximal numbers of antibody-forming cells were found 9 days after a single injection of M. tuberculosis, about 1 day before the appearance of maximal circulating antibody. Immunoelectrophoretic studies and 2-ME studies on 19S and 7S globulin fractions obtained by gel f...

1. The cause for the phenomenon of hemagglutination with heated PVM suspensions has been sought. 2. Evidence in wide variety indicates that the component responsible for hemagglutination is the virus particle itself. 3. The virus is capable of combining with a substance present in lung tissue of certain mammalian host species susceptible to infection by PVM. The occurrence of such combination a...

A complement fixation test modified by the addition of porcine serum and an indirect hemagglutination test were used to detect antibodies to Pseudomonas pseudomallei in pigs. These tests together with cultural examinations were carried out with 250 pigs. The sensitivity and specificity values were 79.3 and 99.5% and 82.8 and 93.2% for the modified complement fixation and hemagglutination tests,...

The diagnostic value of the hemagglutination inhibition test for Newcastle disease has been established for some years (Brandly et al., 1946). The question of the relationship of the hemagglutination inhibition activity (HI) of immune sera to virus-neutralizing activity (SN) or to the refractivity of the host to challenge infection is still one of both fundamental and practical importance. Data...

Serology improves influenza diagnosis by capturing cases missed by reverse transcriptase PCR (RT-PCR). We prospectively evaluated microneutralization and hemagglutination inhibition assays for 2009 influenza A (H1N1) virus diagnosis among 24 RT-PCR-confirmed cases and 98 household contacts. Compared to hemagglutination inhibition, microneutralization demonstrated a higher level of concordance w...

Glutaraldehyde-fixed sheep erythrocytes (GA-SRBC) were used in the indirect hemagglutination test for the detection of Pasteurella antibody. GA-SRBC were stable for at least 6 months. Heat extract or potassium thiocyanate extract antigens of Pasteurella strains could be adsorbed onto GA-SRBC or tanned GA-SRBC, respectively. The indirect hemagglutination test reaction was capsular group specific...