Hysterosonography (SHG) is a new noninvasive technique that involves the slow infusion of sterile saline solution into the uterus during ultrasound imaging. Expansion of endometrial cavity by SHG allows optimal visualization and plays an important role in the evaluation of abnormalities related to the uterus. The most common indications for hysterosonography are: abnormal uterine bleeding both ...

We present a heuristic treatment which relates SHG image intensities, signal directionality, and observed morphology to the physical structure of collagen and cellulose fibrillar tissues. The SHG creation model is based upon relaxed phase matching conditions which account for dispersion, randomness, and axial momentum contributions from the media, and includes a mathematical treatment which rel...

PURPOSE Human corneas with or without stromal edema were examined by second harmonic generation (SHG) imaging microscopy to characterize stromal collagen organization. METHODS Tissue buttons from 31 corneas with stromal edema and 8 normal corneas were fixed, and 3-mm(2) blocks were cut and stained with phalloidin, to visualize the cytoskeleton. The blocks were examined by SHG imaging with a l...

Second harmonic generation (SHG) microscopy is a powerful tool for studying submicron architecture of muscles tissues. Using this technique, we show that the canonical single frequency sarcomeric SHG intensity pattern (SHG-IP) of premetamorphic xenopus tadpole tail muscles is converted to double frequency (2f) sarcomeric SHG-IP in metamorphic climax stages due to massive physiological muscle pr...

Dendritic spines mediate most excitatory inputs in the brain. Although it is clear that spines compartmentalize calcium, it is still unknown what role, if any, they play in integrating synaptic inputs. To investigate the electrical function of spines directly, we used second harmonic generation (SHG) imaging of membrane potential in pyramidal neurons from hippocampal cultures and neocortical br...

This study examines the effect of women-centric agricultural self-help groups (SHGs) on intra-household decision-making in agriculture. Using data collected from an survey 815 households, we analyze women's membership different types SHGs 14 decisions related to farms and households. Specifically, investigate discord decision-making, which is difference perceived roles spouses within a househol...

We theoretically compare the performance of TPEF and SHG microscopy for membrane potential imaging. We argue that electrochromic TPEF and SHG membrane potential responses are reflections of the same phenomenon, and can be described in a unified manner as resulting from the linear Stark effect. We also show that TPEF and SHG exhibit similar sensitivities in the case of both electrochromic and or...

We demonstrate focusing coherent light on a nanoparticle through turbid media based on digital optical phase conjugation of second harmonic generation (SHG) field from the nanoparticle. A SHG active nanoparticle inside a turbid medium was excited at the fundamental frequency and emitted SHG field as a point source. The SHG emission was scattered by the turbid medium, and the scattered field was...

We demonstrate that simultaneous second-harmonic generation (SHG) and two-photon-excited fluorescence (TPEF) can be used to rapidly image biological membranes labeled with a styryl dye. The SHG power is made compatible with the TPEF power by use of near-resonance excitation, in accord with a model based on the theory of phased-array antennas, which shows that the SHG radiation is highly structu...

We study theoretically and experimentally second-harmonic generation (SHG) of 150-fs-duration amplified Ti:sapphire laser pulses at a wavelength of 780 nm in the nonlinear crystal KDP of different lengths and at different power densities as high as 150 GW cm(-2). The experimentally observed SHG conversion efficiency does not exceed 50%. It is shown theoretically that one possible process limiti...