نتایج جستجو برای: hcv genome

تعداد نتایج: 249841  

2014
Kaneez Fatima Esam Azhar Shilu Mathew Ghazi Damanhouri Ishtiaq Qadri

This study described the structural characterization of Pakistani HCV NS3 GT3a in parallel with genotypes 1a and 1b NS3. We investigated the role of amino acids and their interaction patterns in different HCV genotypes by crystallographic modeling. Different softwares were used to study the interaction pattern, for example, CLCBIO sequence viewer, MODELLER, NMRCLUST, ERRAT score, and MODELLER. ...

2012
Tetsuro Suzuki

More than 170 million individuals are currently infected with hepatitis C virus (HCV) worldwide and are at continuous risk of developing chronic liver disease. Since a cell culture system enabling relatively efficient propagation of HCV has become available, an increasing number of viral and host factors involved in HCV particle formation have been identified. Association of the viral Core, whi...

2015
Tzu-Chun Chen Chung-Han Hsieh Peter Sarnow Aleem Siddiqui

The small GTPase Rab27a has been shown to control membrane trafficking and microvesicle transport pathways, in particular the secretion of exosomes. In the liver, high expression of Rab27a correlates with the development of hepatocellular carcinoma. We discovered that low abundance of Rab27a resulted in decreased hepatitis C virus (HCV) RNA and protein abundances in virus-infected cells. Curiou...

2011

The Abbott RealTime HCV Genotype II kit is designed for use in the transcription and amplification of the 5’UTR or NS5B regions of the hepatitis C virus (HCV) genome. RealTime-PCR amplification is conducted in three separate reaction mixtures with different fluorophores where genotypespecific probes are used to detect HCV genotypes 1, 1a, 1b, 2, 3, 4, 5 and 6 such that: • Reaction A detects all...

Journal: :Journal of virology 2010
Takahiro Masaki Ryosuke Suzuki Mohsan Saeed Ken-ichi Mori Mami Matsuda Hideki Aizaki Koji Ishii Noboru Maki Tatsuo Miyamura Yoshiharu Matsuura Takaji Wakita Tetsuro Suzuki

In this study, we used an RNA polymerase I (Pol I) transcription system for development of a reverse genetics protocol to produce hepatitis C virus (HCV), which is an uncapped positive-strand RNA virus. Transfection with a plasmid harboring HCV JFH-1 full-length cDNA flanked by a Pol I promoter and Pol I terminator yielded an unspliced RNA with no additional sequences at either end, resulting i...

Journal: :Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2003
Leon Worth Julian Elliott Jonathan Anderson Joe Sasadeusz Alan Street Sharon Lewin

mining subcellular localization of HCVgenomic RNA in lymphocytes. The aim of our study was merely to determine whether the cervical smear cells obtained from HCV-seropositive women were infected with HCV genomic RNA. We will include the subcellular staining of HCVinfected cells in our continuing study. FISH was performed in accordance with the protocol by Pinkel et al. [3]. The sequence of the ...

Journal: :Journal of virology 2002
Heather J Ezelle Dubravka Markovic Glen N Barber

Hepatitis C virus (HCV), a major etiologic agent of hepatocellular carcinoma, presently infects approximately 400 million people worldwide, making the development of protective measures against HCV infection a key objective. Here we have generated a recombinant vesicular stomatitis virus (VSV), which expresses the HCV structural proteins, by inserting the contiguous Core, E1, and E2 coding regi...

Journal: :The Journal of biological chemistry 2002
Anna Nadal María Martell J Robin Lytle Alita J Lyons Hugh D Robertson Beatriz Cabot Juan I Esteban Rafael Esteban Jaime Guardia Jordi Gómez

We have found that RNase P from HeLa cells specifically and efficiently cleaves hepatitis C virus (HCV) transcripts in vitro. The evidence includes identification of the 5'-phosphate polarity of the newly generated termini at position A(2860) as well as immunological and biochemical assays. Active cleavage has been shown in five dominant sequences of HCV "quasispecies" differing at or near the ...

Journal: :Applied and environmental microbiology 2015
Stephanie Pfaender Janine Brinkmann Daniel Todt Nina Riebesehl Joerg Steinmann Jochen Steinmann Thomas Pietschmann Eike Steinmann

Virus inactivation by chemical disinfectants is an important instrument for infection control in medical settings, but the mechanisms involved are poorly understood. In this study, we systematically investigated the effects of several antiviral treatments on hepatitis C virus (HCV) particles as model for enveloped viruses. Studies were performed with authentic cell culture-derived viruses, and ...

2016
Mojtaba Mortazavi Mohammad Zarenezhad Seyed Moayed Alavian Saeed Gholamzadeh Abdorrasoul Malekpour Mohammad Ghorbani Masoud Torkzadeh Mahani Safa Lotfi Ali Fakhrzad

BACKGROUND The hepatitis C virus (HCV) has six major genotypes. The purpose of this study was to phylogenetically investigate the differences between the genotypes of HCV, and to determine the types of amino acid codon usage in the structure of the virus in order to discover new methods for treatment regimes. METHODS The codon usage of the six genotypes of the HCV nucleotide sequence was inve...

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