Background and objectives: The seed extract of Silybum marianum contains seven flavonolignans known collectively as silymarin. These metabolites can be produced in hairy root cultures of S. marianum. The effect of different physical factors can change root biomass and silymarin production which has been investigated in the present study. Methods<...

Buckwheat, Fagopyrum tataricum Gaertn., is an important medicinal plant, which contains several phenolic compounds, including one of the highest content of rutin, a phenolic compound with anti-inflammatory properties. An experiment was conducted to investigate the level of expression of various genes in the phenylpropanoid biosynthetic pathway to analyze in vitro production of anthocyanin and p...

Maesa lanceolata is a medicinal plant, growing in the tropics of Africa and Asia. It produces a mixture of triterpenoid saponins, some of which have medicinal properties that are of commercial interest. For instance, saponins isolated from Maesa lanceolata exert antiviral, haemolytic and antiangiogenic activities [1]. As conventional plant growth and production is not very well controlled, in v...

Agrobacterium rhizogenes-mediated 'hairy root' cultures were established in Atropa acuminata. The chemical profiling of the hairy roots was carried out by a new mass spectrometric technique, direct analysis in real time (DART). The intact hairy roots were directly analyzed by holding them in the gap between the DART ion source and mass spectrometer. Two alkaloids, atropine and scopolamine, were...

Arabinogalactan protein (AGP), a class of cell wall proteoglycan, was isolated from the hairy root cultures of a newly developed hairy root line IIT-BT/D1 of Catharanthus roseus (L.) G. Don. AGP was found to be present both in the roots (0.3 mg/g fresh weight) and in the spent media (47 mg/L). The compositional analysis revealed the predominance of arabinose and galactose sugar, a characteristi...

Objective: This study involved the formulation of protocol of Agrobacterium rhizogenes mediated transformation for the detailed study of isoflavones (IFs) metabolism in soybean. Methods: Cotyledons were separated from 4-day-old soybean seedlings and infected with three different A. rhizogenes strains under various time durations of sonication, vacuum infiltration and cocultivated on Murashige a...

Gloriosa superba L. a medicinally important monocot, member of the family Liliaceae, was investigated for callusing, direct regeneration and induction of hairy root using soil bacterium Agrobacterium rhizogenes. Friable callus was initiated from corms on basal MS medium amended with 2,4-D (1 mg L) and kinetin (1 mg L). Callus generated from such effective combinations were co-cultivated with th...