cept on the vertical axis is 95. From the data plotted in accordance with equation 1, the values found for the dissociation constant Ki and the inactivation rate constant kinact were 38 ± 4 × 10 M and 1.05 ± 0.08 × 10 sec, respectively. These values compare very favorably with those for mechanismbased inhibitors of phosphatases. As shown in Figure 4, menadione shares critical structural feature...

Background: Escherichia albertii has been recently isolated from the feces of people with gastroenteritis as a pathogen that causes diarrhea. Due to insufficient information on phenotypic and biochemical characteristics E. albertii, it is difficult distinguish other species Enterobacteriaceae family and, therefore, mistakenly identified coli or even Hafnia alvei. Objective: The present study wh...

A 4-h Micro-ID technique for direct identification of oxidase-negative gram-negative rods from positive blood cultures was compared to subculture and species identification of single colonies by API 20E and Micro-ID, using standardized inocula. A total of 127 patients (220 positive cultures) were studied. Isolates included 96 Escherichia coli, 46 Klebsiella pneumoniae, 7 Klebsiella oxytoca, 8 E...

The structural genes for the nickel and cobalt resistance of the conjugative plasmid pEJH 501 of Hafnia alvei 5-5, contained on a SalI-EcoRI fragment of 4.8 kb, were cloned and sequenced. The DNA sequence included five genes in the following order: ncrA, ncrB, ncrC, ncrY, and ncrX. The predicted amino acid sequences of ncrA were homologous to the amino acid sequences of nreB of Achromobacter xy...

Lysine decarboxylase of Escherichia coli has been the subject of enzymological studies, and the gene encoding lysine decarboxylase (cadA) and a regulatory gene (cadR) have been mapped. This enzyme is induced at low pH in the presence of lysine and achieves maximal level under anaerobic conditions. The induction of lysine decarboxylase increases the pH of the extracellular medium and provides a ...