Strain 0083 and two field isolates of H. paragallinarum were previously converted into NAD-independent organisms by the use of crude DNA extractions from naturally occurring NAD-independent H. paragallinarum isolates. Two of these transformed isolates [0083(T) and A745(T)] were used as DNA donors in another transformation experiment in which another field isolate (M85) was used as the DNA recip...

AIMS To determine the nucleotide sequences of adenovirus (Ad) types 1 and 6 fibre genes; to clarify the molecular basis of the distinct haemagglutination properties of subgenus C Ads and their phylogenetic relations. METHODS Human Ad1 and Ad6 fibre genes were sequenced from genomic DNA by direct sequencing. Primer selection was based on alignment of the fibre gene of human Ad serotypes Ad2 an...

In the course of studying the properties of several viruses pathogenic for suckling mice (Stanley and Hayes, 1950; Stanley et al., 1951), it was found necessary to attempt to purify certain strains to determine if virus, free from large amounts of non-virus protein, could be used in haemagglutination or complement-fixation tests. This paper describes the purification procedures adopted with thr...

Two monoclonal antibodies raised against Sindbis virus were shown to be specific for the envelope glycoprotein E1 by radioimmunoprecipitation (RIP). They had a number of contrasting biological properties. One of them was capable of neutralizing virus infectivity and inhibiting haemagglutination, while the other had no significant neutralizing or haemagglutination-inhibiting capability, but did ...

Mukteswar strain of Newcastle disease (ND) virus was propagated in 9-day-old embryonated eggs via allantoic route. The harvested allantoic fluid with haemagglutination (HA) titre of 1024 (EID50 10) was inactivated using 0.1 percent formalin solution. Two experimental vaccines were prepared in light mineral oil with aqueous to oil ratio of 1:2 ( MK-VAC-I) and 1:4(MK-VAC-II).The hydrophile lipoph...

Cytopathic coronaviruses were isolated in HRT-18 cells from bloody faecal samples collected from cows in Québec dairy herds having experienced typical outbreaks of winter dysentery (WD). The formation of polykaryons in the infected cell cultures was found to be dependent on the presence of trypsin in the medium. The WD isolates differed from the prototype Mebus strain of bovine enteropathogenic...