introduction: superovulation produces complications in some patients, so invitro maturation of oocytes is used to decrease or eliminate these complications and improve ivf. moreover, ivm is used for different aspects of reproductive researches. slaughterhouse ovaries are the main source of oocytes for ivm and ivf studies. different media has been introduced and experimented for in vitro maturat...

objective: this study evaluated in vitro maturation (ivm) of oocytes in the germinal vesicle (gv) stage in stimulated intracytoplasmic sperm injection (icsi) cycles. materials and methods: a total of 26 women, aged 18 -37 years, who were candidates for icsi at the fatemeh zahra infertility and health reproductive research center in 2007 were recruited for this study. we used the standard long p...

In Vitro Maturation (IVM) is a method that immature oocytes in antral follicles are extracted and matured in laboratry conditions. This review has attempted to provide the current knowledge and recent findings in in vitro maturation of oocytes and highlights the most important factors involved in this process. The review is based on literature reports and the author's experience. In IVM cycles,...

In micromanipulation experiments using immature oocytes, final ooplasmic maturation is often compromised because the oocytes are usually first freed from their nurturing cumulus cells. This study was undertaken to determine whether cumulus-free in vitro maturation (IVM) in mice could be improved by modifying IVM medium having defined components. Cumulus-free germinal vesicle (GV) stage oocytes ...

Studies were conducted to compare viability of immature buffalo oocytes vitrified in different types of cryoprotectants on the post-thaw morphological appearance, the in vitro maturation and developmental competence of buffalo oocytes. The vitrification solution (VS) consisted of Dulbecco’s phosphate buffered saline (DPBS) supplemented with 0.5 M sucrose, 0.4% bovine serum albumin (BSA) and dif...

Background: Leukemia inhibitory factor (LIF) is a 45-56 kDa glycoprotein that has an important role in proliferation and embryo implantation. Its effect on oocyte maturation and how to exert the function remained to be elucidated. Methods: Immature mice superovulated with human menopausal gonadotropin and germinal vesicle (GV) oocytes were obtained from ovary 48 hours after. GV oocytes were cul...

background   objective:   materials and methods   results   conclusion   key words:   . vitrification, viability, in vitro maturation, gvbd, oocyte, mouse : these results suggest that step-wise vitrification of gvbd oocytes as compared to single step vitrification was better in the rate of survival and in vitro maturation of oocytes. : all non-vitrified oocytes were viable after 24 hr; however,...

BACKGROUND Our aim was to evaluate whether extending the interval between human chorionic gonadotrophin (hCG) priming and immature oocyte retrieval increases the oocyte maturation rate following in vitro maturation (IVM). METHODS This study was performed retrospectively. IVM was performed on 113 polycystic ovary syndrome patients (n = 120 cycles). Oocyte collection was performed either 35 h (...

Background: Different culture media are used for in vitro maturation of ovine oocyte. The Weymouth medium are used for in vitro maturation of rat and mice oocytes with very low levels of supplementation (Just FBS, while to acquire the good results in TCM hormone, and serum supplementation and in some cases growth factor are necessary to gain the optimized results. The aim of the present study w...