نتایج جستجو برای: guanidinium chloride
تعداد نتایج: 88764 فیلتر نتایج به سال:
The collagens were studied in 13 normal and 19 myxomatous human mitral valves. The collagens of the valve were completely solubilized by using a method consisting of guanidinium chloride extraction, limited pepsin digestions and CNBr cleavage of the residue. The normal valves contained 74% type I, 24% type III and 2% type V collagen. The type I and type III collagens had similar solubility patt...
The amino acid sequence of Leishmania mexicana triose phosphate isomerase is unique in having at position 65 a glutamic acid instead of a glutamine. The stability properties of LmTIM and the E65Q mutant were investigated by pH and guanidinium chloride-induced unfolding. The crystal structure of E65Q was determined. Three important observations were made: (a) there are no structural rearrangemen...
Tryptophan oxygenase (L-tryptophan: oxygen oxidoreductase, EC 1.13.1.12) was purified to a state of homogeneity from substrate-induced Pseudomonas acidovorans. The sedimentation and diffusion coefficients of the homogeneous protein are SU),~, 6.26 x lo-l3 set, and DzO w, 4.78 X 10d7 cm2 set-l, and from these values a molecular weight, M,,D, of 121,000 is obtained. High and low speed equilibrium...
1. A compound of hyaluronate and protein, called hyaluronate-protein was isolated from pooled human synovial fluids by caesium chloride density-gradient ultracentrifugation. 2. The isolated hyaluronate-protein was labelled with [(125)I]iodide and the following studies were done. (a) Ultracentrifugation in caesium chloride showed that the protein moiety ((125)I counts) and hyaluronate (hexuronat...
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