Understanding the molecular pathways perturbed in smokeless tobacco- (ST) associated head and neck squamous cell carcinoma (HNSCC) is critical for identifying novel complementary agents for effective disease management. Activation of nuclear factor-kappaB (NF-κB) and cyclooxygenase-2 (COX-2) was reported in ST-associated HNSCC by us [Sawhney,M. et al. (2007) Expression of NF-kappaB parallels CO...

The development of glutamine synthetase (GS) in the neural retina of chick embryos has recently been employed in studies on the control of specific biosynthesis in differentiating cells (Kirk and Moscona, 1963; Moscona and Kirk, 1965). Retinal GS activity is characterized by a sharp increase that begins about the 16th day of embryonic development, continues until after hatching, and coincides w...

background: acanthamoeba-related disease have a poor prognosis according to many previous studies. thus researches regarding biochemical and molecular aspects of this organism are a high priority. to this end achieving high amount of amoebae in culture is the first step for such studies. the main aim of the present research was to address the usage of tyi-s-33 (tripticase, yeast extract, iron-s...

objective: in all protocols for isolation of mesenchymal stem cells (mscs), a few days after culture initiation, the medium were discarded along with its contents of non-adherent cells and the adherent cell population kept and expanded as mscs population. in the present study, attempt was made to expand the cells suspended in removed medium of primary culture and compare them with the adherent ...

the purpose of this research was to design a semi-industrial culture medium to obtain the maximum biomass production from bacterium bacillus subtilis utb96. in the first step, in order to reduce the cost of culture medium components, commercial products and industrial by products including, sugar beet molasses, sugar cane molasses, potato waste water and milk serum as carbon sources and corn st...

OBJECTIVE To analyze the effects of exogenously added glucose (Glc), glucosamine (GlcN) and glucosamine sulfate (GS) on the intracellular UDP-hexoses (UDP-Hex), UDP-N-acetylhexosamines (UDP-HexN) and UDP-glucuronic acid (UDP-GlcA) levels in bovine primary chondrocytes. METHODS Chondrocytes were incubated with different concentrations of Glc, GlcN and GS either in high- or low-glucose DMEM for...

This study was carried out in order to determine the effects of boron and cold stress on pollen germination of almond hybrids in vitro culture, two different media including the absence and presence of boron (0 mg/l, and 100 mg/l) in 10% sucrose and 1% agar medium at 24°C in dark conditions. The results showed that different treatments had a significant effect on the germination percentage. The...

in order to determine the best growing media for gerbera jamesonii under alkaline conditions, a factorial experiment was conducted with two factors, namely: (i) growing medium, and (ii) concentrations of bicarbonate (nahco3) in nutrient solution. results showed that increasing the concentration of nahco3 from 0 to 40 mm in nutrient solution significantly decreased plant growth, maximal quantum ...

The secretion of a glutathione-S-conjugate, dinitrophenyl-glutathione (GS-DNP) was studied in the Caco-2 cells, a cultured human colonic adenocarcinoma cell line with many of the characteristics of enterocytes. The labelled glutathione conjugate was generated within the cell by incubation with 14C-labelled 1-chloro-2,4-dinitrobenzene (CDNB). This compound is hydrophobic and enters the cell by s...