Background: The production of recombinant human growth hormone is usually problematic in Escherichia coli, and achieving higher functional protein yields economically important. Objectives: In this study, the chromosomal expression (hGH) araBAD operon was investigated using Rosetta-gami BL21 (DE3) competent cells under regulation arabinose T7 promoters inducer IPTG, respectively. Subsequently, ...

background in this study, we evaluated the prevalence of the most common mutations occurring in enhancer ii (enhii), basal core promoter (bcp), precore (pc), and core (c) regions of hepatitis b virus (hbv) genome. objectives we also investigated the correlation between hbv variants, their genotypes, and patients’ hbe antigen (hbeag: soluble shape of the capsid antigen) status patients and metho...

Background: ATP-binding cassette membrane transporter G2 (ABCG2) gene is one of transporter family and well characterized for their association with chemoresistance. Promoter methylation is a mechanism for regulation of gene expression. O6-Methyl guanine DNA methyl transferase (MGMT) gene plays a fundamental role in DNA repair. MGMT has the ability to remove alkyl adducts from DNA at the O6 pos...

objective: e-cadherin is widely down-regulated and tightly associated with tumor invasion and metastasis in multiple human cancer types. recent studies have shown that aberrant methylation of the e-cadherin gene promoter contributes to its silencing. however, information regarding epigenetic inactivation of e-cadherin in colorectal cancer is insufficient. herein, we correlate association of the...

This study was conducted to identify polymorphisms in the promoter and coding regions of the bovine growth hormone and growth hormone receptor genes and to study association of polymorphisms identified in these genes with growth traits and serum insulin-like growth factor-I (IGF-I) concentration. The denaturing gradient gel electrophoresis method and sequencing were utilized to identify three n...

Induction of the Pho response in Bacillus subtilis occurs when the P(i) concentrations in the growth medium fall below 0.1 mM, a condition which results in slowed cellular growth followed by entry into stationary phase. The phoPR promoter region contains three sigma(A)-responsive promoters; only promoter P(A4) is PhoP autoregulated. Expression of the phoPR operon is postexponential, suggesting ...