The initial rate of phagocytosis, oxygen consumption rate during phagocytosis, bactericidal capacity against Escherichia coli, and the granule protein composition of isolated mature-appearing granulocytes were studied in 23 patients with chronic granulocytic leukemia (CGL) with the simultaneous use of normal controls. The initial rate of phagocytosis was decreased (p less than 0.05) in the CGL ...

Leukocyte activation is a property of systemic infection. In a previous animal experiment we could demonstrate, that granulocytes, which were already activated by the immune system, were more useful in the evaluation of scintigraphic imaging infection than non activated donor granulocytes. The purpose of the present study was to investigate the in vitro activation of isolated polymorphonuclear ...

Physiological status and litter size affect ewes' hematological parameters, which serve as health indicators. These parameters reflect changes in an animal's physiological condition. Therefore, this study examined the profiles of Awassi ewes with single twin pregnancies during pregnancy postpartum. A total 232 sexually mature healthy (123 109 pregnancies) between ages 3 4 years were included cu...

The effect of different leukocyte antibodies on the fate in vivo of granulocytes is not known. Thus, the optimum in vitro serologic tests to determine a safe and effective granulocyte transfusion or to diagnose immune destruction of granulocytes in other clinical situations have not been identified. We have studied the effect of granulocyte agglutinating (GA), granulocytotoxic (GC), and lymphoc...

AIMS The occurrence of human granulocytic ehrlichiosis (HGE) in a patient with chronic myelogenous leukaemia (CML) provided an opportunity to study whether Anaplasma phagocytophilum, the aetiological agent of HGE, infects mature or immature cells, both in vivo and in vitro. METHODS Diagnosis of HGE was confirmed by culture, polymerase chain reaction (PCR), detection of intragranulocytic inclu...

It has been shown previously that professional phagocytic granulocytes are present in the testis of the gilthead seabream, a seasonal breeding teleost that offers an excellent model for studying the testicular regression process that occurs in seasonal testicular involution and sex change. It is unexpected that testicular granulocytes produce interleukin-1beta, a regulator for spermatogonia pro...

The effects of pyocyanine (phenazinium, 1-hydroxy-5-methyl-hydroxide, inner salt) on oxidative burst in human polymorphonuclear leukocytes were studied by several different approaches. In a cell- and enzyme-free system, pyocyanine oxidized NADPH. The reduced pyocyanine could be measured by its reaction with ferricytochrome c. It was shown by this assay that resting as well as phorbol myristate ...

BACKGROUND Triggering receptor expressed on myeloid cells-1 (TREM-1) amplifies Toll-like receptor-initiated responses against pathogens. We aimed to characterize TREM-1 expression and function during sepsis caused by Burkholderia pseudomallei (melioidosis). METHODS TREM-1 expression was determined on leukocytes and plasma from 34 patients with melioidosis and 32 controls and in mice with expe...

The aim of this study was to compare the strength of the surface adhesion of migrating human granulocytes and the main parameters of their locomotion under the influence of various external forces. The strength of adhesion of granulocytes moving in a quiescent medium was studied by detaching them gravitationally from the plane surface situated above them. In these conditions the force of adhesi...

Myeloperoxidase (MPO) is a major protein present in myeloid cells and is used by these cells to help kill microbes. The human promyelocytic HL-60 line can be induced to differentiate to granulocytes or macrophagelike cells. Poly (A) containing RNA was isolated from HL-60 granulocytes, HL-60 macrophages, HL-60 blasts, and normal human granulocytes. The mRNA was translated in a reticulocyte lysat...