We report the successful outcome of intracytoplasmic sperm injection (ICSI) treatment in two siblings with familial globozoospermia. After controlled ovarian hyperstimulation and oocyte pick-up, retrieved oocytes were mechanically activated before ICSI and a fertilization rate of 33.3% was achieved in the first case. The second couple underwent ICSI without oocyte activation and a 9.1% fertiliz...

Background The aim of this study was to establish a cell-free sequential culture system that can support high levels of in vitro embryo development and blastocyst formation from bovine zygotes. To this end, this investigation was carried out to evaluate the effects of glucose, serum and EDTA on bovine zygote in vitro development. MaterialsAndMethods Bovine presumptive zygotes were derived from ...

Here, we describe a pre-derivation embryo haplotyping strategy that we developed in order to maximize the efficiency and minimize the costs of establishing banks of clinical grade hESC lines in which human leukocyte antigen (HLA) haplotypes match a significant proportion of the population. Using whole genome amplification followed by medium resolution HLA typing using PCR amplification with seq...

During Assisted Reproductive Technology (ART) procedures, the transfer of a single embryo dramatically reduces the rate of multiple pregnancy. Proper information, therefore, should be delivered to patients before embryo transfer. In many countries, regulations limit, according to age of the patient, the number of embryos to be transferred. Selective fetal reduction should not be considered as a...

background: the purpose of the study was to compare clinical pregnancy and delivery rates with fresh and frozen embryo transfer in patients admitted to shiraz- human assisted reproductive center with ovarian hyperstimulation syndrome (ohss). methods: ohss patients randomly divided in two groups, group a (n=50) with fresh embryo transfer and group b (n=50) with frozen embryo transfer. we used vi...

Human sperm assay (HSA) is a preferred in house quality control and proficiency test (PT) practiced in fertility laboratories. HSA is performed over varying durations, apparently without following set criteria. To better understand the assay time required for reprotoxicity testing in embryo culture media, we compared American-Association-of-Bioanalysts-(AAB-) administered HSA data to our own as...

background: embryo selection is a vital part of in vitro fertilization (ivf) programs, with morphology-based grading systems having been widely used for decades. time-lapse imaging combined with embryo morph kinetics may proffer a non-invasive means for improving embryo selection. we report the first ongoing and chemical pregnancies using time-lapse embryo scope to select best embryos for trans...

ABSTRACT Global advances in reproductive biotechnology have allowed for the transfer of embryos from donor females with high genetic merit to recipients using cryopreservation technique, which preserves an embryo excellent quality and viability, thereby achieving a feasible pregnancy rate. The objective this study was evaluate viability Holstein that been cryopreserved more than 40 years under ...

The objective of this guideline from the Canadian Fertility and Andrology Society is to synthesize evidence on preimplantation genetic testing for aneuploidies (PGT-A) using trophectoderm biopsy 24-chromosome analysis provide clinical recommendations Grading Recommendations Assessment, Development Evaluation (GRADE) framework. To date, randomized controlled trials have been limited good-prognos...