Covalent modification of proteins with phosphatidylinositol (PI) was first suggested as a novel mechanism of membrane attachment when it was found that PI-specific phospholipase C (PI-PLC) could release alkaline phosphatase from tissue slices [1-5]. Subsequent compositional analysis of Trypanosoma brucei variant surface glycoprotein (VSG) [6,7], Torpedo acetylcholinesterase [8], erythrocyte ace...

A wide range of proteins are attached to the external face of the plasma membrane of eukaryotic cells via a glycosyl—phosphatidyl-inositol (GPI) anchor (1,2). The function of many GPIanchored proteins is to transport small molecules and ions across the plasma membrane by the process of potocytosis (3). Synthetic GPI-anchored versions of some type I integral membrane proteins and secreted protei...

We determined the acyl and alkyl chain composition of GPI-anchors isolated from MDCK and Fischer rat thyroid (FRT) cells. Both cell lines synthesize GPI-anchors containing C16/C18 or C18/C18 saturated acyl and alkyl chains. The GPI-anchored placental alkaline phosphatase (PLAP) expressed in both cells is raft-associated and PLAP purified from FRT cells is raft-associated in vitro when reconstit...

Autonomous glycosylphosphatidylinositol (GPI) molecules (also protein-free GPIs or free GPIs) have been reported to be particularly abundant in some parasitic protozoa and mediate strong immunomodulatory effects on the host immune system. In the work at hand we have investigated the existence of free GPIs in Babesia bovis. Comparative thin layer chromatographic analysis of the protein-free glyc...

Glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) is abundant in serum and associates with high density lipoproteins (HDL). We have characterized the distribution of GPI-PLD among lipoproteins in human plasma. Apolipoprotein (apo)-specific lipoproteins containing apoB (Lp[B]), apoA-I and A-II (Lp[A-I, A-II]), or apoA-I only (Lp[A-I]) were isolated using dextran sulfate and immunoa...

OBJECTIVE The routine measurement of IgA anticardiolipin (aCL) and IgA anti-β2 glycoprotein I (anti-β2 GPI) antibodies remain controversial despite several studies demonstrating an association with thromboembolic disease in patients with systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS). This controversy may be a contributing factor for the current under use of IgA antiphos...

BACKGROUND Deep brain stimulation (DBS) of the globus pallidus interna (GPi) and subthalamic nucleus (STN) has been reported to relieve motor symptoms and levodopa-induced dyskinesia in patients with advanced Parkinson disease (PD). Although it has been suggested that stimulation of the STN may be superior to stimulation of the GPi, comparative trials are limited. OBJECTIVE To extend our rand...

The need for housing Indonesian people is increasing yearly due to the high population growth rate. This fact can gradually trigger global warming. There a intervention in residential sector minimize resulting negative impacts by developing green building concept. study uses an extended Theory of Planned Behavior identify factors influencing purchase intention and willingness pay (GH) Jabodetab...

A monolithic visible supercontinuum (SC) source with a record average output power of 204 W and spectrum ranging from 580 nm to beyond 2400 is achieved in piece standard telecom graded-index multimode fiber (GRIN MMF) by designing the pumping system. The influence GRIN MMF length on geometrical parameter instability (GPI) effect analyzed for first time, best our knowledge, comparing SC spectral...