Therapeutic uses of DNA functionalized gold nanoparticles (DNA-AuNPs) have shown great potential and exciting opportunities for disease diagnostics and treatment. Maintaining stable conjugation between DNA oligonucleotides and gold nanoparticles under thermally stressed conditions is one of the critical aspects for any of the practical applications. We systematically studied the thermal stabili...

The accurate evaluation of telomerase activity, a typical cancer biomarker, is vital for early screening. In this study, we developed dark-field microscopy (DFM) visual single-particle detection scheme to detect activity based on automatic counting gold nanoparticles (AuNPs). This method started with attaching the substrate (TS) primer magnetic beads (MBs) through streptavidin-biotin interactio...

Gold nanoparticles (AuNPs) have been widely studied in a great mass of cellular biomarkers detection and diagnostics due to their unique combination physical optical properties. Sea urchin-like gold (SUL-AuNPs) are well characterized multi-thorn nanostructures which possess at least four nanothorns on spherical surface, mimicking the morphology sea urchin. Compared AuNPs, SUL-AuNPs showed wide ...

Well-known surface properties of gold nanoparticles (AuNPs) offer easy surface modification with desired biomolecule, thus enabling them to be used for targeting and imaging of cancer cells/tissues. However, targeting and imaging capability come through after synthesis coating of AuNPs' surface with targeting or imaging molecules. Attempts have been made to conjugate both imaging and targeting ...

Gold nanoparticles (AuNPs) have been self-assembled onto electrochemically deposited polyaniline (PANI) films on indium-tin-oxide (ITO) coated glass plates to fabricate glucose biosensor. The covalent immobilization of glucose oxidase (GOx) in the near vicinity of gold nanoparticles has been obtained using N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS), chemist...

The biocompatibility and ease of functionalization of gold nanoparticles underlie significant potential in biotechnology and biomedicine. Eight different proteins were examined in the preparation of gold nanoparticles (AuNPs) in aqueous medium under microwave irradiation. Six of the proteins resulted in the formation of AuNPs. The intrinsic pH of the proteins played an important role in AuNPs w...

To suppress the cytotoxicity of gold nanoparticles (AuNPs), danshensu, a naturally occurring polyphenol antioxidant isolated from Chinese herb, was used to provide a fundamental protection layer for AuNPs, to alleviate oxidative stress and as a reducing agent to react with chloroauric acid. Besides danshensu, gum arabic was chosen as an auxiliary stabilising agent to improve the stability of Au...

By grafting with ethylene diamine tetraacetic acid (EDTA), novel multi-walled carbon nanotube nano-composites (EDTA-MWCNTs) have been successfully prepared. The integration of gold nanoparticles (AuNPs) and novel functionalized matrix shows very promising applications in the fabrication of biosensors for sensitive detection of dopamine in the presence of ascorbic acid and uric acid with the com...

Phytochemicals occluded in tea have been extensively used as dietary supplements and as natural pharmaceuticals in the treatment of various diseases including human cancer. Results on the reduction capabilities of phytochemicals present in tea to reduce gold salts to the corresponding gold nanoparticles are presented in this paper. The phytochemicals present in tea serve the dual roles as effec...

The Au nanoparticle (AuNPs) and DNA oligonucleotide conjugates will have complicated microstructures because one AuNP can be conjugated to more than one single-stranded DNA (ssDNA) on its surface. In this paper, the AuNPs-DNA conjugates with controllable microstructure were prepared on SiO2 microsphere carriers. The reactions between AuNPs with ssDNA on SiO2 carriers were sterically restricted ...