BACKGROUND Decreasing blood glutamate concentrations after traumatic brain injury accelerates brain-to-blood glutamate efflux, leading to improved neurologic outcomes. The authors hypothesize that treatment with blood glutamate scavengers should reduce neuronal cell loss, whereas administration of glutamate should worsen outcomes. The authors performed histologic studies of neuronal survival in...

PURPOSE We successfully developed recombinant human interferon alpha-2b (rhIFN- α 2b) and mutein forms through the site-directed mutagenesis technique. The mutein forms were developed by substituting cysteins at positions 2 and 99 with aspartic acids. The potential adverse effects of these rhIFN- α 2bs were assessed by acute and subchronic studies. METHODS In the acute study, rhIFN- α 2bs wer...

BACKGROUND Pancreatic ductal adenocarcinomas (PDA) activate a glutamine-dependent pathway of cytosolic nicotinamide adenine dinucleotide phosphate (NADPH) production to maintain redox homeostasis and support proliferation. Enzymes involved in this pathway (GLS1 (mitochondrial glutaminase 1), GOT1 (cytoplasmic glutamate oxaloacetate transaminase 1), and GOT2 (mitochondrial glutamate oxaloacetate...

The diagnostic accuracy of two methods for measuring serum glutamic-oxaloacetic transaminase activity was examined in 50 cases of acute myocardial infarction proven by autopsy and in 12 nonfatal cases documented by rigid clinical criteria. All activities measured with the revised spectrophotometric method were elevated during the second day after infarction, and all patients had an elevated act...

Controlled photooxidation inactivates supernatant glutamate aspartate transaminase through the destruction of histidyl residues only. Since previous studies have failed to show alterations in the over-all enzyme structure, the mechanism of transamination has been examined before and after photooxidation, taking advantage of the spectral changes associated with the enzyme prosthetic group, pyrid...

Objectives: In this present study the hepatoprotective effect of an optimized herbal formula (HF) was appraised against paracetamol induced liver damage in rats. Methods: In vitro antioxidant activity HF evaluated by ABTS radical scavenging assay. The (100, 200 and 400 mg/kg b.w) assessed Serum enzymatic levels serum glu...

We have examined a polymorphism for the soluble glutamate oxaloacetate (GOT-1) isozyme system which was found in the Asian mouse Mus castaneus. Variants of GOT-1 segregate as though they are controlled by codominant alleles for a single autosomal locus which we have designated Got-1. No close linkage of genes for soluble and mitochondrial forms of the enzyme, GOT-1 and GOT-2 respectively, was o...

Use of improvised explosive devices has significantly increased the incidence of traumatic brain injury (TBI) and associated neuropsychiatric deficits in the recent wars in Iraq and Afghanistan. Acute deleterious effects of single and repeated blast exposure can lead to long-term neurobiological effects and neuropsychiatric deficits. Using in vitro and in vivo shock tube models of blast-induced...

BACKGROUND Garcinia spp. belongs to the family Clusiaceae has been traditionally used for the treatment of many ailments including the liver damage. Garcinia dulcis found in North Eastern region of Assam; India can be a potential candidature to combat different ailments. Objective: The present work has been designed in such a way to appraisal the antioxidant and hepatoprotective activity of fru...