background: activated normal platelets undergo many biochemical and morphological changes, some of which are apoptotic. platelet derived microparticles and shrinked platelets as hallmark of platelet activation and apoptosis disperse surfaces containing procoagulant activity around injured vessels and tissues. this study was conducted to determine microparticles formation and platelet shrinkage ...

background: the aim of this study was to detect the prognostic significance of survivin level and the expression of total p53 in acute lymphoblastic leukemia (all) and its correlation to patients' outcome. methods: sixty two children newly diagnosed with acute lymphoblastic leukemia were treated with chemotherapy and followed up for 2 years or until death. twenty apparently healthy volunteers w...

fragrant species of the genus salvia have been attributed many medicinal properties, which include anticancer activity. in the present study, cytotoxic properties of total methanol extract of salvia chloroleuca rech. f. & aellen and its fractions were investigated on mcf-7, a breast carcinoma cell line. malignant and non-malignant cells were cultured in rpmi medium and incubated with different ...

background: recent researches have demonstrated that human t-cell immunoglobulin mucin 1 (tim-1) glycoprotein plays important roles in regulation of autoimmune and allergic diseases, as well as in tumor immunity and response to viral infections. therefore,targeting tim-1 could be a potential therapeutic approach against such diseases.objectives: in this study, we aimed to express tim-1 protein ...

background  the mammary gland in humans is a dynamic organ that undergoes significant developmental changes during pregnancy, lactation, and involution. stem cells derived from human breast milk possess the adult stem cell-like characteristics such as self-renewal, proliferative and differentiate potential. this source of stem cells avoids invasive procedures and the ethical controversy of embr...

Background: Several studies have demonstrated the immunosuppresive effects of mes-enchymal stem cells (MSCs) in allogeneic or mitogenic interactions. Cell-cell contact inhibition and secretion of suppressive soluble factors have been suggested in this re-gard. Objective: To investigate if adipose derived MSCs could inhibit Jurkat lym-phoblastic leukemia T cell proliferation during coculture. Me...

Despite the toxic effects on numerous organs, silver nanoparticles can be used to cease cell cycle and promotes apoptosis in cancerous cells. Therefore, by choosing the right dosage nanoparticles can be helpful for cancer treatment, whilst do not harm normal tissues. In the current study, it has been tried to measure silver nanoparticles toxicity against HPB-ALL leukemia cell line and normal hu...

Despite the toxic effects on numerous organs, silver nanoparticles can be used to cease cell cycle and promotes apoptosis in cancerous cells. Therefore, by choosing the right dosage nanoparticles can be helpful for cancer treatment, whilst do not harm normal tissues. In the current study, it has been tried to measure silver nanoparticles toxicity against HPB-ALL leukemia cell line and normal hu...

Flow cytometry, initially developed to analyze surface protein expression in hematopoietic cells, has increased in analytical complexity and is now widely used to identify cells from different tissues and organisms. As a consequence, data analysis became increasingly difficult due the need of large multi-parametric compensation matrices and to the eventual auto-fluorescence frequently found in ...

Quantitative cell state measurements can provide a wealth of information about mechanism of action of chemical compounds and gene functionality. Here we present a comparison of cell cycle disruption measurements from commonly used flow cytometry (generating onedimensional signal data) and bioimaging (producing two-dimensional image data). Our results show high correlation between the two approa...