The aim of this study was to detect class Ⅰ, II and III integrons using multiplex PCR, and to analyze the role that these integrons play in mediating multidrug-resistant Staphylococcus aureus (SA). The sensitivity of SA to 20 types of antibiotics was examined using the K-B method. A genomic DNA extraction kit was used for extracting genomic DNA an...

a total of ten isolates of fungi with rhizoctonia-like mycelia were obtained from infected roots and stems of pistachio pistacia vera grown in a commercial nursery in rafsanjan, iran, during the autumn of 2011. the infected seedlings showed symptoms of chlorosis that later turned to necrosis. all of the isolates were identified as binucleate rhizoctonia on the basis of hyphal characteristics an...

In this article, we present the genomic DNA yield and the microsatellite and single nucleotide polymorphism (SNP) genotyping success rates of genomic DNA extracted from a large number of mouth swab samples. In total, the median yield and quality was determined in 714 individuals and the success rates in 378,480 genotypings of 915 individuals. The median yield of genomic DNA per mouth swab was 4...

High-quality mRNA extraction is essential for gene expression assays. In this study, we developed a rapid method (20 min) named FACTS the of intact RNA from Pseudomonas aeruginosa and compared its performance to established techniques like RNAsnap CiAR. The integrity, yield, purity presence residual genomic DNA were adopted as assessment criteria. Multiple assays integrity applied, including Ag...

background: cystic hydatid disease is an important zoonosis, affecting humans and animals and is a significant public health and economic problem throughout the world and iran. since extraction of dna from the parasite is a primary and crucial step which has a principal effect on pcr results, in the current study five simple methods for dna extraction from protoscoleces of echinococcus granulos...

We attempted to determine the number of sperm cells required for genotyping of one microsatellite marker. The crude genomic DNA extracted from about 760 or more sperm cells gave sufficient quantity of PCR product using a 20 microl-scale PCR. We also studied the effects of non-ionic detergents on extraction of crude sperm genomic DNA. PCR products amplified with the crude sperm genomic DNA extra...

We describe a rapid and cost-effective technique for the in vitro removal of introns and other unwanted regions from genomic DNA to generate a single sequence of continuous coding capacity, where tissues required for RNA extraction and complementary DNA synthesis are unavailable. Based on an overlapping fusion-PCR strategy, we name this procedure SPLICE (for swift PCR for ligating in vitro cons...

INTRODUCTION The purity of genomic DNA (gDNA) extracted from different clinical specimens optimizes sensitivity of polymerase chain reaction (PCR) assays. This study attempted to compare two different DNA extraction techniques namely salting-out and classic phenol-chloroform. MATERIALS AND METHODS Qualification of two different DNA extraction techniques for 634 clinical specimens highly suspe...