adenoviruses are used extensively to deliver genes into mammalian cells, particularly where there is a requirement for high-level expression of transgene products in cultured cells, or for use as recombinant viral vaccines or in gene therapy. in spite of their usefulness, the construction of adenoviral vectors (adv) is a cumbersome and lengthy process that is not readily amenable to the generat...

b-lactoglobulin (coded by the b-lg gene) is the major milk whey protein in ruminants. studies have shown that this protein is polymorphic in many breeds of sheep as a result of a single base pair substitution in the b-lg gene that also gives rise to rsai restriction fragment length polymorphism (rflp). blood samples were collected from 391 animals belonging to 5 iranian and 6 russian sheep bree...

bovine leukemia provirus: evidence of presence of part of gag gene inseminal plasma of naturally infected bullsit is of critical importance to understand the modalities of blv presence in semen, especially with regard to artificial insemination (ai). presence of bovine leukemia provirus was demonstrated in fresh and frozen semen samples by researchers. in this study paired blood and semen sampl...

Background and Aims: lamivudine is amongst the antiviral for drug chronic hepatitis B treatment. During therapy with lamivudine, variants may emerge with YMDD mutation in the reverse transcriptase (RT) region of polymerase gene. This mutation might have a role in drug resistant for HBV. Materials and Methods: HBV DNA extraction from serum sample of 88 patients, were subjected to nested PCR for ...

The standard method for comparing gene products (proteins or RNA) is to compare their DNA or amino acid sequences. Additional information about some gene products may come from multiple sources, including the set of Gene Ontology (GO) annotations and the set of journal abstracts related to each gene product. Gene product similarity measures can be based on evaluating sets of descriptor terms fo...

the growth hormone (gh) gene is a candidate for growth trait in farm animals and plays an important role in growth metabolism. growth hormone can contribute in growth metabolism when it's receptor (ghr) are on cells target. the single nucleotide polymorphism was occurred in gh and ghr genes and that associated with carcass traits in lori-bakhtiari sheep breed with using pcr-sscp. in this r...

Advances in gene therapy have led to a wide range of products. Hence, it is challenging use standard non-clinical safety tests, which are typically used evaluate chemically synthesized Therefore, case-by-case evaluation based on the unique characteristics each product necessary. In Japan, fundamental considerations for evaluating products outlined “Ensuring Quality and Safety Gene Therapy Produ...