The geminiviral replication-associated protein (Rep) is the only viral protein required for viral DNA replication. Tomato leaf curl virus (TLCV) Rep was expressed in Escherichia coli as a histidine-tagged fusion protein and purified to homogeneity in non-denaturing form. The fusion protein was used in in vitro binding experiments to identify the Rep-binding elements within the origin of replica...

ABSTRACT Cucurbit leaf curl virus (CuLCV), a whitefly-transmitted geminivirus previously partially characterized from the southwestern United States and northern Mexico, was identified as a distinct bipartite begomovirus species. This virus has near sequence identity with the previously partially characterized Cucurbit leaf crumple virus from California. Experimental and natural host range stud...

The CRISPR/Cas9 system has recently been used to confer molecular immunity against several eukaryotic viruses, including plant DNA geminiviruses. Here, we provide a detailed analysis of the efficiencies of targeting different coding and non-coding sequences in the genomes of multiple geminiviruses. Moreover, we analyze the ability of geminiviruses to evade the CRISPR/Cas9 machinery. Our results...

For the nuclear replicating bipartite geminiviruses such as squash leaf curl to systemically infect the host requires the active participation of two virus-encoded movement proteins, BR1 and BL1. These act in a cooperative manner to transport the viral singlestranded DNA genome from i ts site of replication in the nucleus to the cell periphery (A.A. Sanderfoot, S.C. Lazarowitz [1995] Plant Cell...

The genome of the geminivirus tomato golden mosaic virus (TGMV) consists of two single-stranded circular DNAs, A and B, that replicate through a rolling-circle mechanism in nuclei of infected plant cells. The TGMV origin of replication is located in a conserved 5' intergenic region and includes at least two functional elements: the origin recognition site of the essential viral replication prot...

Tomato leaf curl New Delhi virus (ToLCNDV, family Geminiviridae, genus Begomovirus) is a whitefly-transmitted that causes widespread damage in Cucurbitaceae and Solaneceae crops worldwide. The Spanish strain, ToLCNDV-ES, affects mainly cucurbit has spread through the Mediterranean basin since its first detection 2013 south of Spain. control been based on adoption measures to vector, which have ...

Tomato leaf curl Bangalore virus (ToLCBV) (Geminiviridae) causes the economically important tomato disease (ToLCVD), and is transmitted by whitefly, Bemisia tabaci (Gennadius) (Aleyrodidae: Hemiptera). Successful transmission of insect requires safe translocation through different barriers inside vector. During translocation, viral coat protein will interact with many whitefly proteins, while a...

The silverleaf whitefly (Bemisia tabaci) is actually a complex of morphologically indistinct species that vary in their capacity to transmit plant viruses and to induce physiological disorders in plants of economic importance. The worldwide impact of this whitefly has increased greatly, as it is a vector of various types of phytovirus, especially geminiviruses, in plants of economic impor...

Whitefly-transmitted geminiviruses are major pathogens of the important crop cassava in Africa. The intensive sampling and sequencing of cassava mosaic disease-causing viruses that occurred in the wake of a severe outbreak in Central Africa (1997-2002) allowed us to estimate the rate of evolution of this virus. East African cassava mosaic virus and related species are obligately bipartite (DNA-...

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