Pulsed field gel electrophoresis (PFGE), the gold standard of molecular typing methods, has a major disadvantage of an unusually long electrophoretic time. From the original protocol of 6 days, it was modified to 3 days and subsequently to a single day. We describe the procedure of stacking five to six gels one on top of another in order to increase and maximize the output in a shorter time wit...

The objective of this study was to produce a Carbopol 940 based gel formula containing an Azadirachta indica leaf extract and evaluate its wound healing potential. ethanolic derived from the dried leaves subjected phytochemical evaluation. Three formulations in three different concentrations, i.e., 1, 2, 3% w/w were prepared. These gels evaluated for their physical appearance, stability, antimi...

BACKGROUND AND AIMS We hypothesized that the i-gel™ supra-glottic airway can be inserted with relative ease in a reversed manner just like a Guedel's airway. MATERIAL AND METHODS A prospective, randomized, controlled interventional trial was conducted on 100 patients to compare reversed insertion of the i-gel™ (Group R) with the conventional insertion (Group C). In Group C, i-gel™ was introdu...

BACKGROUND Both the i-gel™ (i-gel) and LMA Supreme™ (Supreme) are new single-use second generation supraglottic airway devices available in pediatric sizes. This study was designed to investigate the i-gel in comparison with the Supreme in children undergoing general anesthesia. METHODS One hundred children with American Society of Anesthesiologists physical status I or II undergoing general ...

Proteins labeled with a luminescent lanthanide chelate, {{2,2',2'',2'''-{4'-{[(4,6-dichloro-1,3,5-triazin-2-yl)amino]biphenyl-4-yl}-2,2':6',2''-terpyridine-6,6''-diyl}bis(methylenenitrilo)}tetrakis(acetato)}europium(III) (DTBTA-Eu(3+)),were analyzed with sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) slab gel electrophoresis and hydroxyethyl cellurose gel capillary electrophoresis with a ...

PURPOSE To investigate the penetration of 0.3% gatifloxacin ophthalmic gel, 0.3% gatifloxacin ophthalmic solution and 0.5% levofloxacin ophthalmic solution into aqueous humor after topical application. MATERIALS AND METHODS Age-related cataract patients (150 eyes in 150 cases) receiving phacoemulsification were randomly divided into three groups: a 0.3% gatifloxacin gel group (n=50), a 0.3% g...

The influence of extracellular matrix configuration on the tissue-specific function of cultured hepatocytes was investigated. Adult rat hepatocytes sandwiched between two layers of collagen gel were compared to cells cultured on a single layer of collagen gel for differences in the total RNA content, the level of albumin-specific mRNA, the rate of albumin gene transcription, and the rate of alb...

An understanding of pressure propagation mechanisms is crucial in finding suitable conditions for the temporary storage, transmission and attenuation a large signal multi-plug gelled pipeline. The current work investigates transient flow start pipeline, filled with weakly compressible, thixotropic waxy crude oil. A gel can form either naturally or artificially. We solve time-dependent mass mome...

artemisinin extracted from artemisia annua l. is the best medicine with the highest efficiency, the most effective and the lowest toxicity in treating ague nowadays. at present, most studies focus on artemisinin and its derivatives, while the study and report about the other active components are rare. this paper purposed to further discover new indication of artemisia annua l. connecting with ...