Aging cells not only cease growing, but also secrete various proteins such as inflammatory cytokines. This secretory phenomenon is known the senescence‑associated phenotype (SASP). The aim of present study was to elucidate effects senescence on differentiation osteoclast precursors (OCPs) and corresponding SASP. RAW264.7 were used OCPs cultured passage (P)5, P10 P20. Cell proliferation assays, ...

BACKGROUND Gc protein has been reported to be a precursor of Gc protein-derived macrophage activation factor (GcMAF) in the inflammation-primed macrophage activation cascade. An inducible beta-galactosidase of B cells and neuraminidase of T cells convert Gc protein to GcMAF. MATERIALS AND METHODS Gc protein from human serum was purified using 25(OH)D3 affinity column chromatography and modifi...

Vinyl sulfone (VS)–agarose beads were used to develop a new strategy coimmobilize enzymes with very different stabilities, enabling the reuse of most stable immobilized enzyme. Two model combi-biocatalysts prepared. First, trypsin and chymotrypsin multipoint covalently coimmobilized on VS–agarose, support was blocked ethylenediamine, then β-galactosidase from Aspergillus oryzae via anion exchan...

BACKGROUND/AIMS We demonstrated the role of caveolin-1 involved in high glucose (HG)-induced glomerular mesangial cells (GMCs) senescence. METHODS HG was used to stimulate GMCs. The telomere lengths were analyzed by Southern blot. β-Galactosidase staining was determined. The expressions of caveolin-1 and P53 proteins were determined by Western blot. RESULTS Treatment with high concentration...