نتایج جستجو برای: fusion pcr
تعداد نتایج: 291966 فیلتر نتایج به سال:
Background A specific chromosomal abnormality, the Philadelphia chromosome (BCR-ABL fusion), is present in all patients with chronic myeloid leukemia (CML). The b2a2 and b3a2 fusion mRNAs encode p210 fusion protein p210 and e1a2 encode p190. The aim of this study was to evaluate the frequency of BCR-ABL fusion transcript variants in Northeast of Iranian CML patients and to compare the laborator...
BACKGROUND Sequence variations in glycoproteins of influenza virus surface impel us to design new candidate vaccines yearly. Ectodomain of influenza M2 protein is a surface and highly conserved protein. M2e in influenza vaccines may eliminate the need for changing vaccine formulation every year. OBJECTIVES In this study, a recombinant baculovirus containing M2e and cholera toxin subunit B fus...
Abstract Transgenic plants expressing immunoglobulin (Ig) M Fc-fused Prostate acid phosphatase (PAP) antigenic proteins (PAP-IgM FcK) and J-chain were generated by Agrobacterium-mediated transformation. The Fc region was tagged with the ER retention motif (KDEL) to make PAP-IgM FcK. Two transgenic crossed together generate F 1 both FcK × J-chain). PCR RT-PCR analyses confirmed transgene inserti...
Background: Subunit vaccines are appropriate vaccine candidates for the prevention of some infections. In this study, three immunogenic proteins of Mycobacterium tuberculosis, including HspX, Ppe44, and EsxV as a new construction, were expressed alone and as a fusion protein to develop a new vaccine candidate against tuberculosis infection. Methods: To make the fusion protein, the three genes ...
The aim of this study was to construct, expression of a novel recombinant chimeric protein consisting of Pyruvate dehydrogenase beta subunit (PDHB) and high antigenic region of integral membrane lipoprotein P80 of Mycoplasma agalactiae as a potential diagnostic tool. The full-length sequence of pdhb and a portion of antigenic regions of P80 were selected and analyzed by CLC ma...
A DNA construct encoding cholera toxin B subunit (CTB) and an anti-diabetic peptide from human heat shock protein 60 (hsp60) as a fusion protein was produced and transferred into low-nicotine tobacco through Agrobacterium-mediated transformation. Integration of the fusion gene into the plant genome was confirmed by PCR. The transcriptional expression of the fusion gene was demonstrated by RTPCR...
Cancer is one of the major causes of mortality in Ecuador and annually, hematological malignancies are within the top ten most common cancers. In this multicentric study, we analyzed a series of patients diagnosed with different hematological disorders between the years 1984 and 2012. Chromosome abnormalities were detected in 1886 (45.9%) patients. FISH and RT-PCR techniques were used in order ...
a generalization of the known results in fusion frames and $g$-frames theory to continuous fusion frames which defined by m. h. faroughi and r. ahmadi, is presented in this study. continuous resolution of the identity (cri) is introduced, a new family of cri is constructed, and a number of reconstruction formulas are obtained. also, new results are given on the duality of continuous fusion fram...
abstract background: spondylolysis and spondylolisthesis can be associated with significant low back pain, especially in physically active adolescents. non-operative management is usually successful in improving symptoms, but surgical intervention is occasionally required. the aim of this study was to determine the effect of in situ posterolateral fusion in the treatment of refractory case...
One case of paediatric Ewing's sarcoma and two peripheral primitive neuroectodermal tumours/extra-osseous Ewing's sarcoma were studied for the characteristic t(11;22) translocation, using a recently described RNA-polymerase chain reaction method (RNA-PCR). PCR products of the expected sizes were obtained from RNA derived from the Ewing's sarcoma and the peripheral primitive neuroectodermal tumo...
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