UNLABELLED Similar to many eukaryotic viruses (and unlike bacteriophages), viruses infecting archaea are often encased in lipid-containing envelopes. However, the mechanisms of their morphogenesis and egress remain unexplored. Here, we used dual-axis electron tomography (ET) to characterize the morphogenesis of Sulfolobus spindle-shaped virus 1 (SSV1), the prototype of the family Fuselloviridae...

This topic with the examples of budding bear series guided by sustainable design, in three dimensional animation role comprehensive analysis comparison, induction and summary, hope through design to promote application development, under artistic expression innovation concept, for study provide theoretical basis. article first 3 d animation, example, role, introduce bud content, were discussed ...

The budding yeast YCS4 gene encodes a conserved regulatory subunit of the condensin complex. We isolated an allele of this gene in a screen for mutants defective in sister chromatid separation or segregation. The phenotype of the ycs4-1 mutant is similar to topoisomerase II mutants and distinct from the esp1-1 mutant: the topological resolution of sister chromatids is compromised in ycs4-1 desp...

in order to study the effect of rootstock, scion and some methods of grafting and budding to achieve the best methods of grafting in pomegranate, split factorial experiment was conducted in a completely randomized design in march 2013 in greenhouse at the agricultural college of vali-e-asr university of rafsanjan. factors included rootstock, scion, and methods of grafting and time of grafting. ...

Although considerable progress has been made towards characterizing virus assembly processes, assignment of the site of tegumentation and envelopment for human cytomegalovirus (HCMV) is still not clear. In this study, we examined the envelopment of HCMV particles in human lung fibroblasts (HF) HL 411 and HL 19, human umbilical vein endothelial cells, human pulmonary arterial endothelial cells, ...

Secretory proteins are exported from the endoplasmic reticulum (ER) at specialized regions known as the transitional ER (tER). Coat protein complex II (COPII) proteins are enriched at tER sites, although the mechanisms underlying tER site assembly and maintenance are not understood. Here, we investigated the dynamic properties of tER sites in Saccharomyces cerevisiae and probed protein and lipi...