The aim of this study was to determine the effect of gamma-oryzanol-enriched -rice bran oil on the quality of cryopreserved boar semen. Ten boars provided semen of proven motility and morphology for this study. The semen was divided into three portions in which lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with 2 types of rice bran oils, at a ga...

This experiment wascarried out to explore the effect of use of combinations of permeating cryoprotectant and non permeating cryoprotectant on thequality of frozen-thawed Beluga (Huso huso) spermas compared to the use of permeating cryoprotectant as a control. For this work methanol (MeOH) and dimethyl sulfoxide (DMSO)are used aspermeatingcryoprotectantsand bovine serum albuminas a non permeatin...

OBJECTIVE To determine the effect of semen storage and separation techniques on sperm DNA fragmentation. DESIGN Controlled clinical study. SETTING An assisted reproductive technology laboratory. PATIENT(S) Thirty normoozospermic semen samples obtained from patients undergoing infertility evaluation. INTERVENTION(S) One aliquot from each sample was immediately prepared (control) for the ...

The purpose of this study was to investigate the efficiency of the water test to evaluate the functional membrane integrity of ejaculated canine sperm after collecting, cooling, and freezing-thawing. Twenty-five ejaculates were obtained from 12 stud dogs by digital manipulation. Sperm-rich fractions were evaluated, diluted, cooled at 5oC and frozen-thawed. A conventional hypo-osmotic swelling t...

Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphol...


 This study was designed to evaluate the supplementation of various concentrations selenium and vitamin E combinations in lactose egg-yolk-glycerol based extender on cryosurvival buffalo bull semen. The semen four healthy donor bulls ejaculates (n= 80) were processed pooled. collected extended with experimental extenders then frozen. frozen straws thawed analyzed for motility, viability, ...

This study determines the effect of different commercial diluents on quality frozen Simmental semen. The extraction fresh semen from 3 bulls using an artificial vaginal method was performed twice a week. Fresh with motility >70% further processed to one-step dilution method, and used were Andromed®, Optixcell®, Steridyl®. diluted packed into mini-straws, equilibrated, frozen, stored for test...

To investigate the possibility to carry pathogen bacteria in turkey flocks via cryopreserved semen, research was carried out 1) to investigate the microbial contamination of fresh and frozen thawed turkey semen and 2) to evaluate the effect of the freezing-thawing process on the survival of 3 serovars of Salmonella spp. experimentally inoculated in turkey semen. Five pools of semen diluted 4-fo...

Female moths of Bombyx mori were artificially inseminated with cryopreserved semen. The fertility of inseminated females varied from 0% to 76.9% depending on the strain. Addition of fresh semen from triploid males, which are infertile but whose semen includes intact apyrene sperm, greatly improved fecundity of cryopreserved semen from normal males. Frozen apyrene sperm from the triploid donors ...

the aim of this study was to compare the effects of using different levels of royal jelly in semen extender on viatal characteristics of goat sperm after freezing. semen was collected from four adult bucks twice a week and samples pooled together. sperm samples (six repeats) containing royal jelly in four levels (0.5, 1, 1.5 and 2%) had been frozen. after semen samples freezing – were thawed an...