The aim of our research was to examine the ability of density gradient preparation BoviPure and swim up method on bull sperm separation and in vitro embryo production (IVP) systems. Frozen/thawed semen from six Simmental bulls was pooled and treated using both methods. The sperm motility, concentration, membrane activity, membrane integrity and acrosomal status were evaluated and compared befor...

The matter of ‘abandoned embryos’ arises when surplus IVF embryos are frozen and stored for later use. If the fertility clinic or storage facility in question does not have clear direction about what to do with these embryos, and/or payment for storage ceases, and/or the embryo providers cannot be reached, the embryos raise an ethical and practical challenge. On the one hand, there is a commitm...

Repeat-breeder (RB) cows are a major source of economic waste due to their decreased fertility. Embryo transfer (ET) is an alternative tool to improve the fertility of RB cows. The aims of the present study were to evaluate the effects of recipient parity and the season on pregnancy rates following ET in RB Japanese Black beef cattle. Embryos were transferred nonsurgically to recipients, consis...

Over a 26 month period 17% of couples having treatment in our clinical programmes selected a commercially available protein (normal serum albumin, NSA) prepared from pooled human sera instead of using their own serum as a supplement for their embryo culture media. In a retrospective analysis of >2000 gonadotrophin-stimulated cycles and 1000 cycles where frozen/thawed embryos were transferred, f...

Aim To evaluate whether local endometrial injury would improve the pregnancy rates after frozen-thawed blastocyst transfer in cycles with hormone replacement. Methods Seventy-seven patients who were aged <40 years with a history of at least three unsuccessful embryo transfers and who underwent frozen-thawed blastocyst transfer during hormone replacement cycles between February and June, 2013 ...

Aim Several studies have indicated that the cause of the increased birthweight of frozen-thawed embryos was associated with assisted reproductive technology (ART) procedures, such as cryopreservation. In the present study, the mean birthweight of singletons was compared between the ovulatory and hormone replacement therapy (HRT) cycles in order to investigate the primary factor that leads to hi...

This qualitative study sought to provide an in-depth understanding of the experiences of couples who have relinquished their stored frozen embryos to one or more infertile couples. All couples were recruited through a U.S. based not-for-profit, prolife, Christian "embryo adoption" agency. In-depth semistructured interviews were undertaken via e-mail. Implications for practice strategies, includ...

Background: The objective of this study is to evaluate whether low-dose aspirin affects doppler sonographic parameters of the uterine arteries, endometrial thickness and pregnancy rate of women undergoing frozen-thawed embryo transfer cycles. Materials and Methods: This randomized, double- blind, placebo-controlled trial study was conducted between April 2012, and March 2013. A total of 60 elig...

background: the purpose of this study was to determine the optimal endometrial preparation protocol by comparing the clinical outcome of two methods of endometrial preparation in frozen-thawed embryo transfer (fet) cycles, including that is, oral estradiol and 17ß-estradiol transdermal patch. methods: in this randomized controlled trial, women underwent either conventional ivf or intracytoplasm...

Background: Vitrification and warming of blastocysts is an established procedure for the preservation of embryos during IVF. Vitrification is so successful that it allows for elective single embryo transfer (eSET) to be performed routinely, since the frozen embryos will be available with high implantation potential if the fresh embryo fails. Comprehensive chromosome screening (CCS) is also a pr...