The quality of excavated and unloaded rock masses on steep high slopes in cold regions is prone to deterioration, which turn affects the long-term stability safety slopes. Based a triaxial unloading-damage test sandstone, unloading quantity was used analyze evolution law damage; freeze-thaw cycle unloaded-damaged samples carried out, it found that average change porosity reloading peak strength...

The relative drying rate of samples (RDR), which is the ratio of the drying rate of pretreated samples to that of untreated ones, might be used as a tool to investigate the damage to cells of agroproducts induced by high-pressure treatment. Damage to cells induced by high pressure was estimated by comparing the RDR after high-pressure pretreatment with the RDR after chloroform-vapor, heat, and ...

Recently, numerous studies of aggregate response to freezing have been reported. Aggregate stability has usually been inversely proportional to soil water content at the time of freezing (Bullock et al., 1988; Benoit, 1973; Bryan, 1971; Logsdail and Webber, 1959). Aggregates from poorly aggregated soils, however, are more stable when frozen at intermediate water contents (Mostaghimi et al., 198...

Ectotherms overwintering in temperate ecosystems must survive low temperatures while conserving energy to fuel post-winter reproduction. Freeze-tolerant wood frogs, Rana sylvatica, have an active response to the initiation of ice formation that includes mobilising glucose from glycogen and circulating it around the body to act as a cryoprotectant. We used flow-through respirometry to measure CO...

Little information is available about the precise mechanisms and determinants of freeze resistance in baker's yeast, Saccharomyces cerevisiae. Genomewide gene expression analysis and Northern analysis of different freeze-resistant and freeze-sensitive strains have now revealed a correlation between freeze resistance and the aquaporin genes AQY1 and AQY2. Deletion of these genes in a laboratory ...

The degradation of concrete material under multiple freeze–thaw cycles is an important issue for structures in cold and wet regions. This paper proposed a physical and mechanical model to explain the deformation behavior observed in previous experiments, from internal pressure calculation to mesoscale simulation, and for both closed and open freeze–thaw tests. Three kinds of internal pressures ...

An enzyme immunoassay producing either a chromogenic or fluorogenic end product was developed and evaluated for detecting La Crosse viral antigen within mosquito pools. The enzyme immunoassay was found to be sensitive, detecting one infected mosquito within a pool of 100 mosquitoes, and specific, distinguishing between closely related California group viruses. Assays were completed within 5 h a...

Exposing fully cold-acclimated wheat plants to a mild freeze-thaw cycle of -3 °C for 24h followed by +3 °C for 24 or 48 h results in dramatically improved tolerance of subsequent exposure to sub-freezing temperatures. Gene enrichment analysis of crown tissue from plants collected before or after the -3 °C freeze or after thawing at +3 °C for 24 or 48 h revealed that many biological processes an...

Sperm storage via freezing has been useful for men who have difficulty masturbating during assisted reproductive technology (ART) programs and before impotency caused by chemotherapy, vasectomy, and other procedures. Studies were undertaken to evaluate the extent of cryoinjury to sperm after repeated freezing and thawing. The results showed that normozoospermic and oligozoospermic sperm survive...