Incubation of erythrocytes with the sulfhydryl reagent N-ethyl-maleimide (NEM) results in altered spectrin self-association and formation of dimers on the membrane. Skeletons isolated from these cells exhibit marked skeletal instability. In addition, NEM treatment induces increased thermal sensitivity of both cells and purified spectrin. These effects were not produced in aerobically incubated ...

A wide variety of molecular mechanisms have been proposed to explain the actions of the synthetic plant growth regulator maleic hydrazide (3). One of the most prominent of these theories holds that MH2 acts as a sulfhydryl reagent (7), and more recently it has been suggested that MH acts as a carbonyl reagent (12). The idea that MH might react with sulfhydryl groups was supported by reports tha...

Blankenship, L. C. (Eastern Utilization Research and Development Division, Washington D.C.), and M. J. Pallansch. Differential analysis of sulfhydryl and disulfide groups of intact spores. J. Bacteriol. 92:1615-1617. 1966.-Fluorescence quenching of fluorescein mercuric acetate in alkaline medium (1 n NaOH) was found to be an accurate, sensitive method for differential analysis of sulfhydryl and...

One sulfhydryl group was modified per mol of native human gastric lipase after incubation at pH 8.0 with 5,5'-dithiobis(2-nitrobenzoic acid) for 18 h or with 4,4'-dithiopyridine for 100 min. With both reagents a direct correlation was found between the modification of one sulfhydryl group and the loss of human gastric lipase activity. Incubation of human gastric lipase with a new hydrophobic su...

The effect of rhodium(II) acetate, propionate, and methoxyacetate on the activity of 17 enzymes was evaluated. The enzymes were preincubated with the rhodium(II) complexes in order to detect irreversible inhibition. All enzymes that have essential sulfhydryl groups in or near their active site were found to be irreversibly inhibited. Those enzymes without essential sulfhydryl groups were not af...

the role of zinc (zn) in enhancing defense capacity of several plants against salinity has been demonstrated but there is limited information on the impact of zn nutrition on alleviating salinity-induced oxidative damage in olive. one-year-old seedlings of two varieties of olive (olea europaea l. cvs. frontoio and conservolea) supplied with three zn levels (0, 1 and 5 mm in the form of znso4.7h...

In the second experiment as reported in the previous chapter, an anti cataract activity of Captopril against oxidant stress induced cataract was investigated attributed to its antioxidant properties. The antioxidant property of Captopril is greatly influenced by the presence of thiol group in its structure which helps to interact with lens specific enzymes and proteins as well. After absorption...

Egg albumin in the native, unaltered state, does not give tests characteristic of sulfhydryl groups. When, however, this protein is treated in any one of several ways, such as by heat (8, 9, 17), ultraviolet irradiation (lo), shaking (lo), or by solution in urea or other amides (7), free sulfhydryl groups make their appearance. The amount of free -SH groups appearing in egg albumin through the ...