Lantadenes are pentacyclic triterpenoids present in the leaves of the plant Lantana camara. In the present study, in vitro antioxidant activity and free radical scavenging capacity of lantadene A was evaluated using established in vitro models such as ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picryl-hydrazyl (DPPH•), hydroxyl radical (OH•), nitric oxide radical (NO•), superoxide ...

in the present study, free-radical-scavenging and antioxidant activities of angipars™, an herbal extract which has been registered as a therapeutic drug for diabetic foot ulcer, were examined. we investigated the protective effect of angipars™ on cytotoxicity produced by exposure to h2o2 (300 µm) in hepg2 and a549 cell lines, using mtt assay, glutathione (gsh) content analysis, and lipid peroxi...

The present investigation highlights the free radical scavenging activity of Melochia corchorifolia, Sida acuta and Saccharum officinarum leaf extracts and their Phenolic contents by determining DPPH, Nitric oxide, Hydroxyl and Hydrogen peroxide scavenging activity. The shade dried leaves were coarsely powdered and subjected to fractional extraction by using chloroform and ethanol. The extracts...

Rat liver and intestinal microsomes were exposed to various free radical generating systems and their effect were assessed by studying different parameters such as formation of malonaldehyde (MDA) and conjugated diene, arachidonic acid depletion and alteration in protein thiol groups and tocopherol levels. These studies revealed that liver being highly vulnerable tissue showed all the effects o...

Under physiological and pathological conditions, the reactive oxygen species (ROS) are formed from both exogenous and endogenous sources. ROS are implicated in a wide number of disease processes, including atherosclerosis, autoimmune disorders, rheumatoid arthritis, neuronal degeneration, cardiovascular diseases and cancer. Among the reactive oxygen species produced in living cells, hydroxyl ra...

The cytotoxic effects of ferric nitrilotriacetate (Fe-NTA) have been considered to be caused by free radicals produced by the drug. The present study was carried out to determine whether or not cytotoxic effects of Fe-NTA on cell growth and lipoperoxide formation of Chinese hamster cells were reduced by antioxidants. Using a spin trapping technique, we found that hydroxyl radical formation in t...

1878-3317/$ e see front matter Copyright 2011, Ta doi:10.1016/j.jecm.2011.04.006 Background: Coumarin is a well-known plant-derived natural product, which is extensively used as a biologically active compound. Coumarins that possess hydroxyl groups in their benzenoid ring were shown to have potent antioxidant and radical-scavenging properties in various experimental models. Purposes: During our...

Spin trapping, a sensitive and specific means of detecting free radicals, is optimally performed on cell suspensions. This makes it unsuitable for the study of adherent endothelial cell monolayers because disrupting the monolayer to induce a cell suspension could introduce confounding factors. This problem was eliminated through the use of endothelial cells that were grown to confluence on micr...

Using the electron spin resonance/spin trapping system, 4-pyridyl 1-oxide N-tert-butylnitrone (4-POBN)/ethanol, hydroxyl radical was detected as the alpha-hydroxyethyl spin trapped adduct of 4-POBN, 4-POBN-CH(CH3)OH, from phorbol 12-myristate 13-acetate-stimulated human neutrophils and monocytes without the addition of supplemental iron. 4-POBN-CH(CH3)OH was stable in the presence of a neutroph...