An original method which uses in vitro anaerobic incubation at 37 degrees C followed by centrifugation, ultrafiltration, and ion exchange chromatography is described; it shows that faecal material suspended in physiological saline can destroy added creatinine. The rate of breakdown by suspensions from uraemic subjects (mean 780 mumol h-1kg-1 SEM 70) was slightly faster than in normal subjects (...

sperm selection techniques have became an important tool to improve sub fertile ejaculates. the main aim of this study was to assess the dynamics of motile sperm subpopulations of ejaculates from subfertile stallions subjected to density gradient centrifugation (dgc) involving a double layer colloid [dgc2], before and after storage for 24 and 48 hours. ejaculates from eight stallions with ferti...

Four techniques for the detection and quantification of Dirofilaria immitis microfilariae in peripheral blood were compared, namely the conventional thick smear, thick films prepared by cytocentrifugation, filtration of blood through polycarbonate membranes, and density gradient centrifugation followed by membrane filtration. The efficiency of the methods and their sensitivity was assessed by d...

objectives lipid components of spermatozoa have an important role in its functional activity. in this study the concentration of cholesterol, phospholipid, triacylglycerol and total lipid in different subsets of human spermatozoa, isolated as three fractions by a discontinuous puresperm gradient, was determined. methods aliquots of the liquefied semen samples (n=107) were layered on top of the ...

A case of myelofibrosis related to thorotrast administration is described. Current concepts of acute myelofibrosis are discussed, as are the varied complications of thorotrast.

612 JEADV 2006, 18, 602–634 © 2006 European Academy of Dermatology and Venereology in the diagnosis of invasive pulmonary aspergillosis. Am J Med 1996; 100: 171–178. 6 Verweij PE, Meis JFGM. Microbiological diagnosis of invasive fungal infection in transplant recipients. Transpl Infect Dis 2000; 2: 80–87. 7 Mennink-Kersten MA, Donnelly JP, Verweij PE. Detection of circulating galactomannan for ...

langerhans cell histiocytosis (lch) is a rare neoplasm defined as the proliferation of bone marrow langerhans cells, which is a kind of dendritic cells. the major pathological features of lch are expression of cd1a and s100 as well as birbeck granules. its presentation can differ from a mild bone lesion to a multi-systemic evolved malignant neoplasm; however, the latter outcome is almost rare. ...