A flow cytometric method for the quantitation of reticulocytes was refined for routine laboratory use. Blood (2 microliters) is added to 2 ml of 0.4 microM thiazole orange in phosphate buffered saline, incubated at room temperature for 90 minutes, and analysed on a Coulter EPICS Profile flow cytometer, with gating for red cells on the basis of forward and right angled light scatter. Blood (2 mi...

The standard benchtop flow cytometer is very similar to a hematology cell counter. In fact, flow cytometers can trace their origins back to the early hematology counters. Unlike their earlier counterparts that used electronic impedance to measure particles in a fluid stream, today’s modern flow cytometers use an illuminating light source usually consisting of a laser or arc lamp. The majority o...

This article reviews recent developments in microfluidic impedance flow cytometry for high-throughput electrical property characterization of single cells. Four major perspectives of microfluidic impedance flow cytometry for single-cell characterization are included in this review: (1) early developments of microfluidic impedance flow cytometry for single-cell electrical property characterizati...

RNA was less studied by flow cytometry (FCM) in comparison to other cellular components. Moreover, most of these studies were applied to the measurement of total RNA. Nevertheless, such a quantification, essentially by the use of acridine orange, but also other dyes or dye combinations, permitted the acquisition of important information about the general metabolism, and the characterization of ...

We report a simplified flow cytometer design that makes use of negative dielectrophoresis (DEP) for particle focusing and integrated optical and AC impedance detectors to enable an inexpensive, compact and robust system for cell and particle characterization. This straightforward, modular design could be applied as a standalone instrument or as a particle detector in an integrated micro total a...

Cell function assays have been redefined over the past several years, largely because of technologies such as flow cytometry. The major difference between other techniques and those proposed using flow cytometry is the ability to analyze viable, single cells in a rapid and quantitative fashion. One of the advantages of flow cytometry methods lies in the ability to obtain population information....

2 Introduction This document demonstrates the functionality of the flowType package for phenotyping FCM assays. flowType uses a simple threshold, Kmeans, flowMeans or flowClust to partition every channel to a positive and a negative cell population. These partitions are then combined to generate a set of multi-dimensional phenotypes. For more details on how this package can be used in a complet...

Fluorescent proteins are now a critical tool in all areas of biomedical research. In this article, we review the techniques required to use fluorescent proteins for flow cytometry, concentrating specifically on the excitation and emission requirements for each protein, and the specific equipment required for optimal use.