abstract background and objective: a high level of homogeneity observed within all bacteria in the mycobacterium tuberculosis complex makes a property that seriously challenges traditional biochemical-based identification methods of these pathogens in the laboratory. the work presented here was conducted to characterize mycobacterium tuberculosis complex isolates in golestan, northern iran. mat...

Bacterial flagellin is important for intestinal immune homeostasis. Flagellins from most species activate Toll-like receptor 5 (TLR5). The principal bacterial food-borne pathogen Campylobacter jejuni escapes TLR5 recognition, probably due to an alternate flagellin subunit structure. We investigated the molecular basis of TLR5 evasion by aiming to reconstitute TLR5 stimulating activity in live C...

abstract        typing of bacteria is an important part of epidemiological studies on nosocomial infections. bacterial identification methods have dramatically improved in recent years, which is mainly due to advancements in the field of molecular biotechnology. in many cases, molecular techniques have replaced phenotypic typing methods. currently, a wide range of bacterial typing techniques is...

Currently, ten-finger typing skills have become the general standard for prospective employees who will apply jobs. These are not only needed IT-based jobs but also in other office. Unfortunately, until now, many students still do these skills. Therefore, training at Nurul Jadid Islamic Boarding School is very much needed. The method used implementation of this activity a practice-based using T...

Using the PCR technique, the presence of the seven genotypic virulence markers of Campylobacter has been tested. It was found that all C. coli (92 strains) and C. jejuni (105 strains) possessed the flaA and cadF markers. Several strains were positive for the CDT toxin genes (76.6%, 85.3%, and 83.2% strains with the A, B, and C cdt gene variants, respectively). On the other hand, only 66.0% and ...

Campylobacter jejuni infection is a leading bacterial cause of gastroenteritis and a common antecedent leading to Gullian-Barré syndrome. Our previous data suggested that the RNA-binding protein CsrA plays an important role in regulating several important phenotypes including motility, biofilm formation, and oxidative stress resistance. In this study, we compared the proteomes of wild type, csr...

Response to changes in light conditions involves a variety of receptors that can modulate gene expression, enzyme activity and/or motility. For the study of light-regulated effects of Agrobacterium tumefaciens, we used a global analysis approach - proteomics - and compared the protein patterns of dark- and light-grown bacteria. These analyses revealed a significant reduction of FlaA and FlaB - ...

Campylobacter jejuni is the most common zoonotic bacterium associated with human diarrhea, and chickens are considered to be one of the most important sources for human infection, with no effective prophylactic treatment available. We describe here a prophylactic strategy using chitosan-DNA intranasal immunization to induce specific immune responses. The chitosan used for intranasal administrat...