We have isolated and characterized a S. pombe promoter using a functional heterologous gene product assay. Random S. pombe genomic fragments were cloned upstream from the promoterless 'lacZ gene and tested in vivo for their efficiency to promote expression of the beta-galactosidase protein in the fission yeast. An efficient S. pombe promoter called 54/1 was isolated and shown to drive up to 5% ...

The yields and velocity distributions of heavy residues and fission fragments from the reaction of 20 MeV/nucleon 197Au + 90Zr have been measured using the MSU A1200 fragment separator. A bimodal distribution of residues is observed, with one group, resulting from peripheral collisions, having fragment mass numbers A= 160–200 while the other group, resulting from “hard” collisions, has A = 120–...

Neutronics-processing interface parameters have large impacts on the neutron economy and transmutation performance of an aqueousbased Accelerator Transmutation of Waste (ATW) system. A detailed assessment of the interdependence of these blanket neutronic and chemical processing parameters has been performed. Neutronic performance analyses requi’re that neutron transport calculations for the A’T...

The cdc25+ gene of fission yeast encodes a phosphotyrosine phosphatase that dephosphorylates tyrosine-15 of p34cdc2 and thereby activates p34cdc2/cyclin to bring about entry into M phase. We have recently cloned a human homolog, CDC25, which rescues the M-phase initiation defect of yeast cdc25 temperature-sensitive mutants. Antibodies raised against the CDC25 gene product specifically recognize...

The ability to separate fission products by electrodeposition from molten salts depends, in part, on differences between the interactions of the different fission product cations with the ions present in the molten salt "solvent". These differences may be expressed as ratios of activity coefficients, which depend on the identity of the solvent and other factors. Here, we demonstrate the ability...

Fission-product gamma-ray line pairs sensitive to fissile material and neutron energy R.E. Marrs a, , E.B. Norman , J.T. Burke , R.A. Macri , H.A. Shugart , E. Browne , A.R. Smith d a Lawrence Livermore National Laboratory, Livermore, CA 94551, USA b Department of Nuclear Engineering, University of California, Berkeley, CA 94720, USA c Department of Physics, University of California, Berkeley, ...

Portioning and Transmutation is the separation and fissioning of the minor actinides and some long-lived fission product from the spent nuclear fuel. In this review, the impact of partitioning and transmutation of nuclear waste based on radiotoxicity, geological repository, proliferation concern and the technological requirement to be fully implemented has been discussed. A summary of the advan...