In this issue of Blood, Grubor and colleagues investigate genomic changes in a large series of patients with CLL. They report novel genetic lesions, ascertain the frequency of losses and gains of genomic areas with greater accuracy, track down candidate genes, and analyze intraclonal heterogeneity. D espite its phenotypic homogeneity, chronic lymphocytic leukemia (CLL) is clinically heterogeneo...

BACKGROUND Fluorescence in situ hybridization (FISH) analysis can provide important information in the management of patients with hematologic malignancies. However, FISH performed in addition to G-banded karyotype can be labor-intensive and expensive. The aim of this study was to evaluate whether FISH gives additional information in the setting of adequate conventional cytogenetics in cases of...

The 45S and 5S rDNA loci were physically mapped by double-probe FISH on mitotic metaphase chromosomes in the six strains of F. esculentum tataricum. Results showed that tataricum very small similar. diploid karyotype formulas these two species 2n=2x=16=12m+4m (SAT) 2n=2x=16=12m+4sm (SAT), respectively. Four pairs IS, IIS, IIIL, VL, as well IL IVS esculentum, five VIIS, three IL, IVS, VIS There ...

Chemicals may induce both numerical and structural aberrations. In addition to these chromosomal mutations, chemicals may render cells genetically unstable, which may result in chromosomal instability. For a detailed analysis, sophisticated approaches at single cell resolution are needed. Such approaches have become feasible by recent developments in molecular cytogenetics. In particular, new m...

Inoculation with bacterial or fungal antigens that stimulate cell proliferation has been widely used to obtain metaphases for cytogenetic studies of fish. We evaluated the potential of new pharmaceutical compounds as mitogenic agents in fish, testing the efficacy of Aminovac (mixed antigens and epsilon-acetamidocaproic acid), Broncho-Vaxom (bacterial lysate) and Estimoral (bacterial lysate) to ...