A soil bacterium capable of degrading chitin on chitin agar plates was isolated and identified as Bacillus pumilus isolate U5 on the basis of 16S rDNA sequence analysis. In order to optimize culture conditions for chitinase production by this bacterium, a two step approach was employed. First, the effects of several medium components were studied using the Plackett-Burman design. Among various ...

A soil bacterium capable of degrading chitin on chitin agar plates was isolated and identified as Bacillus pumilus isolate U5 on the basis of 16S rDNA sequence analysis. In order to optimize culture conditions for chitinase production by this bacterium, a two step approach was employed. First, the effects of several medium components were studied using the Plackett-Burman design. Among various ...

AbstractESW as a natural byproduct, although non-hazardous and contains calcium, magnesium carbonate and protein, is commonly disposed in landfills without any pretreatment because it was traditionally useless. In this regards, we wish to report the synthesis of a novel CaO/Ca2Fe2O5 nanocomposite based on ESW. At the first, eggshell (ES) nanopowder was prepared by ball-milling. Then, the ...

This paper studies the kinetics of the reaction between NaClO3, FeSO4, and NaHSO3, which can potentially be used as an alternative to the conventional lime-limestone process for flue gas desulfurization. The key for the establishment of a kinetic model of the reaction is to find a way to determine concentrations of reactants or products during the reaction. The generation rate of Clduring the r...

BACKGROUND Reactive oxygen species (ROS) are important mediators in a number of degenerative diseases. Oxidative stress refers to the imbalance between the production of ROS and the ability to scavenge these species through endogenous antioxidant systems. Since antioxidants can inhibit oxidative processes, it becomes relevant to describe natural compounds with antioxidant properties which may b...

We investigated the effect of lipid peroxidation, in vitro induced by H2O2 or FeSO4 and ascorbic acid, on binding properties of muscarinic receptors in rat cerebral cortex membranes. Simultaneously the concentrations of thiobarbituric acid reactive substances (TBARS) were measured to assess the extent of lipid peroxidation. In conditions of increased TBARS levels the density of (3H)N-methylscop...

Cell-free extracts prepared by sonication of Streptomyces clavuligerus cyclized 6-(L-aaminoadipyl)-L-cysteinyl-D-valine (ACV) into a penicillin-type antibiotic. The antibacterial spectrum of this antibiotic suggested it was a mixture of isopenicillin N and penicillin N indicating that both cyclization and racemase activities were present. Cyclization activity was optimal in extracts prepared fr...

In studies on the nutritional requirements of Bacillus subtilis during mycobacillin formation in complex media, it was observed that the addition of a mixture of salts (KH2PO4, MgSO4, FeSO4, and MInSO4) to a casein hydrolyzateglucose medium greatly enhanced mycobacillin formation (AIajumdar and Bose, 1959). Reports by a number of workers (Jansen and Hirschman, 1944; Hendlin, 1949) indicate that...

PURPOSE The aim of this study was to investigate the autofluorescence (AF) of the RPE with two-photon microscopy (TPM) and fluorescence lifetime imaging (FLIM) under normal and oxidative stress conditions. METHODS Porcine RPE-choroid explants were used for investigation. The RPE-choroid tissue was preserved in a perfusion organ culture system. Oxidative stress was induced by laser photocoagul...