Biogas adalah gas yang dihasilkan dari proses fermentasi bahan organik dalam kondisi tertutup (anaerob) oleh mikroorganisme. Dalam penelitian ini, produksi biogas menggunakan baku kotoran sapi. Kotoran sapi merupakan sumber baik karena mengandung bakteri penghasil metana. Tujuan ini untuk mengetahui pengaruh penambahan EM4 terhadap kualitas dihasilkan. Penelitian dilakukan di Laboratorium PLC, ...

The present investigation deals with the kinetics of submerged extracellular lipases fermentation by both wild and mutant strains of Rhizopus oligosporus var.microsporus in a laboratory scale stirred fermentor. Other parameters studied were inoculum size, pH, agitation and rate of aeration. It was found that the growth and lipases production was increased gradually and reached its maximum 9.07±...

Bacillus licheniformis ATCC 9945A was grown on Medium E in batch fermentations in which the pH was maintained at 5.5., 6.5, 7.4, and 8.25. The effects of pH on cell growth, carbon source utilization, and gamma-polyglutamic acid (gamma-PGA) production, molecular weight, and polymer stereochemistry were determined. The gamma-PGA yield was highest (15 g/L, 96 h growth time) at pH 6.5. The increase...

BACKGROUND Our laboratory has reported a strategy for improving the extracellular production of recombinant proteins through co-expression with Thermobifida fusca cutinase, which increases membrane permeability via its phospholipid hydrolysis activity. However, the foam generated by the lysophospholipid product makes the fermentation process difficult to control in a fermentor. Phospholipase C ...