BACKGROUND Industrial processes for recombinant protein production challenge production hosts, such as the yeast Pichia pastoris, on multiple levels. During a common P. pastoris fed-batch process, cells experience strong adaptations to different metabolic states or suffer from environmental stresses due to high cell density cultivation. Additionally, recombinant protein production and nutrient ...

A novel optimal control algorithm is presented for application to batch processes. The algorithm utilises a linear model of the process that is identified using multi-way partial least squares. This linear model is used within an optimal control law to regulate the productivity of the batch by manipulating the substrates that are fed into the fermentation vessel. The proposed control algorithm ...

Unbleached pulp from the pulp and paper industry was used as a cellulosic material source to produce bioethanol in a separate hydrolysis and fermentation process. In the batch enzymatic saccharification, three cellulolytic enzymes and different initial carbohydrate (CH) contents were tested. Cellic CTec2 was shown to be the most efficient enzyme with the lowest dosage used: 35 FPU gCH . Sugar p...

Batch and fed-batch cultivation of Bacillus thurillgiellsis var. kurstaki was carried out to achieve high cell and spore density with maximum insecticidal activity. Cell cultivation was done in fully equipped fermentors under controlled conditions at different feed rates. Increased cell mass, high spore counts and improved insecticidal activity were achieved with fed-batch cultivation system. W...

A novel high-density yeast propagation system has been developed, which produced that performed as well cropped in commercial brewery trials. This process is capable of producing concentrations 13 times greater than traditional approaches used breweries to date. The based on a controlled fed-batch fermentation, can produce pitching little 24 h. demonstrator plant was installed regional and from...

BACKGROUND Bioprocess development of recombinant proteins is time consuming and laborious as many factors influence the accumulation of the product in the soluble and active form. Currently, in most cases the developmental line is characterised by a screening stage which is performed under batch conditions followed by the development of the fed-batch process. Performing the screening already un...

Clavulanic acid (CA) is a potent inhibitor of β-lactamases, enzymes that are responsible for the hydrolysis of β-lactam antibiotics. It is a secondary metabolite produced by the filamentous aerobic bacterium Streptomyces clavuligerus in submerged cultivations. In the present work clavulanic acid production in batch, fed-batch and continuous bioreactors was studied with the objective of increasi...

Bioprocesses are appreciated as difficult to control because their dynamic behavior is highly nonlinear and time varying, in particular, when they are operating in fed batch mode. The research objective of this study was to develop an appropriate control method for a complex bioprocess and to implement it on a laboratory plant. Hence, an intelligent control structure has been designed in order ...

A comparative study of software sensors using Multiway Partial Least Squares and Extended Kalman Filters in an application to a fed-batch yeast fermentation process is presented. The MPLS theory is introduced firstly and then applied to a yeast fed-batch fermentation process to provide soft-sensing facilities. The soft-sensing capabilities of the MPLS approach are found to compare favourably wi...