Fluorescence correlation spectroscopy (FCS) represents one of the most sensitive fluorescence techniques due to single molecule detection. Microrheology serves as a tool to study viscoelastic properties of microenvironment of observed particles. We used 100 nm latex particles to study mean square displacement (MSD) of these particles in Newtonian fluids. The comparison of MSD determined by FCS ...

To evaluate the effect of conditioned media (CM) and Extracellular Vesicles (EVs) derived from bovine oviduct epithelial cell (BOEC) lines on the developmental capacity of bovine zygotes and the quality of embryos produced in vitro, presumptive zygotes were cultured under specific conditions. In experiment 1, zygotes were cultured either on monolayers from BOEC extended culture (E), together wi...

Adult erythroid progenitors produce significantly higher fetal hemoglobin (HbF) levels in cultures containing fetal calf serum (FCS) and erythropoietin (Ep) than in vivo. The precise mechanisms for this increased HbF production in culture have not been elucidated. We examined HbF biosynthesis by enriched human progenitors in serum-free (SF) culture. We measured globin chain biosynthesis by comb...

The implementation of finite-control-set model predictive control (FCS-MPC) in voltage source inverters (VSIs) can make the system suffer from poor current harmonics performance, which may complicate design required AC filter. To overcome this shortcoming, a carrier-based modulated (CB-MMPC) strategy is proposed paper. This method enables utilization existing PWM modulation techniques with FCS-...

A substantial amount of rice grains is lost along the value chain, contributing to food insecurity among farming households in Benin. While losses are inevitable it imperative determine minimum acceptable loss for rice. This study aims quantify and its effect on security using Food Consumption Score (FCS) Linear model. The results show a positive low rate FCS. In addition, increases probability...

Institutional change has resulted in the separation of organizations for the protection of animals and children. This project reunites two organizations to examine associations between human violence and animal cruelty. For 12 months, Family and Children's Services (FCS) investigators and Humane Society (HS) investigators in Wellington County, Canada, completed checklists to examine connections...

Fluorescence correlation spectroscopy (FCS) is a powerful spectroscopic technique for studying samples at dilute fluorophore concentrations down to single molecules. The standard way of data acquisition, at such low concentrations, is an asynchronous photon counting mode that generates data only when a photon is detected. A significant problem is how to efficiently convert such asynchronously r...

Fluorescence Correlation Spectroscopy (FCS) is a very popular method to determine the diffusion coefficient of molecules in solution. However, the information contained in a FCS curve can be further exploited: As the mean number of molecules in the confocal detection volume Veff is inversely proportional to the amplitude of the correlation function at very short correlation times G (τ ≈ 0),...

Fetal chondrocytes (FCs) have recently been identified as an alternative cell source for cartilage tissue engineering applications because of their partially chondrogenically differentiated phenotype and developmental plasticity. In this study, chondrocytes derived from fetal bovine cartilage were characterized and then cultured on commercially available Cytodex-1 and Biosilon microcarriers and...

OBJECTIVE To test whether statistical learning on clinical and laboratory test patterns would lead to an algorithm for Kawasaki disease (KD) diagnosis that could aid clinicians. STUDY DESIGN Demographic, clinical, and laboratory data were prospectively collected for subjects with KD and febrile controls (FCs) using a standardized data collection form. RESULTS Our multivariate models were tr...