The discovery of activated protein C (APC) resistance by Dahlbäck in 19931 was a milestone in thrombophilia research that led to major advances in our understanding of the biochemistry, genetics, and clinical manifestations of hypercoagulability. APC resistance was quickly demonstrated to be caused by a single point mutation (1691G3A) in the coding region of the factor V gene.2 This mutation, f...

Objective: to evaluate the impact of Leiden mutation on the course of severe acute pancreatitis. Subjects and methods. One hundred and twelve people were examined. Group 1 comprised 50 patients diagnosed with severe acute pancreatitis with out coagulation factor V (Leiden) mutation. Group 2 included 42 patients with severe acute pancreatitis who were found to have Leiden mutation. Acute pancrea...

We read the article by Ozturk et al. [1], in which they reported the frequency of some thrombophilic mutations in eastern Turkey. The authors have defined single nucleotide gene variations of factor V Leiden, prothrombin G20210A, and methylenetetrahydrofolate reductase (MTHFR) C677T as thrombophilic mutations. However, as current scientific data do not support an increased risk of thrombosis in...

from peripheral blood leukocytes as described previously (5). Amplification of genomic DNA was performed by PCR as described (1). The G3 A substitution at nucleotide position 20210 was detected by allele-specific restriction digestion by using HindIII (1). Among the 27 patients, 4 (14.8%) were heterozygous for the G 20210 3 A mutation in the prothrombin gene and 1 patient (3.7%) was homozy-gous...

Venous thrombosis is a common disease, with an estimated annual incidence in the general population of 1 in 1000 persons (1). It is a major medical problem, which may cause death due to pulmonary embolism. Fibrinogen is a key component of the common clotting pathway; it is involved in the final phases of coagulation. It is also the coagulation factor whose plasma concentration is the highest (2...