Unprimed (CBA X C57BL/6)F1 lymph node T cells were transferred with sheep erythrocytes (SRC) into heavily irradiated F1 or parental strain mice and recovered from thoracic duct lymph or spleens of the recipients 5 days later. To study their helper function, the harvested F1 T cells were transferred with antigen into irradiated F1 mice plus B cells from either the two parental strains or from F1...

The rate of ATP hydrolysis under multi- and unisite conditions was determined in the native F1-inhibitor protein complex of bovine heart mitochondria (Adolfsen, R., MacClung, J.A., and Moudrianakis, E.N. (1975) Biochemistry 14, 1727-1735). Aurovertin was used to distinguish between hydrolytic activity catalyzed by the F1-ATPase or the F1-inhibitor protein (F1.I) complex. We found that incubatio...

Identifying the genes required for environmental sex determination is important for understanding the evolution of diverse sex determination mechanisms in animals. Orthologs of Drosophila orphan receptor Fushi tarazu factor-1 (Ftz-F1) are known to function in genetic sex determination. In contrast, their roles in environmental sex determination remain unknown. In this study, we have cloned and ...

background: major histocompatibility complex (mhc) in chicken has profound influence on resistance/susceptibility to disease, and production and reproduction traits. microsatellite marker lei0258 is a genetic indicator for mhc haplotypes. recognizing diversity of mhc haplotypes in selectively bred populations will be helpful for selecting population resistant to disease and development of effec...

Previous studies have shown that mucosal application of interleukin-12 (IL-12) can stimulate elevated secretory immunoglobulin A (IgA) responses. Since possible exposure to plague is via Yersinia pestis-laden aerosols that results in pneumonic plague, arming both the mucosal and systemic immune systems may offer an added benefit for protective immunity. Two bicistronic plasmids were constructed...

The effect of specific anti-I-J reagents on macrophage-T cell interactions was studied in an in vitro antibody response to burro erythrocytes. Macrophages were prepared from the spleens of F1 hybrid mice whose parental strains differed at the I-J subregion. Two F1 hybrids were used for these experiments, [B10.A(3R) X B10.A(5R)]F1 and [B10.S(9R) X B10.HTT]F1. F1 macrophages responded equally wel...

To investigate the possible enhancing effect of the H-2z haplotype of the New Zealand White (NZW) strain on the production of autoantibodies and renal disease observed in B/W F1 mice, we developed the ZWD/8 strain, a NZW congenic line carrying the H-2d haplotype, produced (NZB X ZWD/8)F1 (B/WD8 F1) mice, and examined the difference in several immunological abnormalities between the B/W F1 (H-2d...

This paper presents a longitudinal analysis of the extent to which age affects F0 and formant frequencies. Five speakers at two time intervals showed a clear effect for F0 and F1 but no systematic effects for F2 or F3. In two speakers for which recordings were available in successive years over a 50 year period, results showed with increasing age a decrease in both F0 and F1 for a female speake...

This article describes how a mixture of two densities, f0 and f1, may be decomposed into a different mixture consisting of three densities. These new densities, f+, f−, and f=, summarize differences between f0 and f1: f+ is high in areas of excess of f1 compared to f0; f− represents deficiency of f1 compared to f0 in the same way; f= represents commonality between f1 and f0. The supports of f+ ...